Research Professor, Department of Microbiology
Professor Emeritus of Microbiology, Department of Microbiology
In collaboration with NYU School of Medicine colleague Dr. G.J. Thorbecke, our group seeks to elucidate the function of IgD, an immunoglobulin that, while in trace amounts in serum (less than 1% of total), is present on the surface of most B lymphocytes. Although IgD's precise biological function has not been defined, considerable evidence indicates its role in humoral immunoaugmentation. In vivo and in vitro murine studies suggest that IgD's augmentation involvement applies to primary and secondary antibody responses, with helper T cells acting as mediators of the effect. This augmentation appears to result from direct contact between IgD and a specific class of T cells expressing IgD receptors on their cell surface (T-delta cells). Such contact causes up-regulation of the IgD receptors on these T cells, allowing them to bind more strongly to B cells and facilitating B-cell activation and proliferation. In microdissection studies of purified murine and human IgD to determine the minimum molecular entity that can induce IgD receptor up-regulation on T cells, we showed that IgD immune augmentation resides solely in the carbohydrate moieties on the molecule and, in murine IgD, specifically in N-linked sequences. Continuing studies examine 1) the precise oligosaccharide sequence(s) responsible for this IgD biological activity, 2) the synthesis of such sequences, 3) the nature and specificity of the IgD receptor, and 4) the use of purified IgD, or carbohydrate sequences therefrom, to stimulate various immune responses.
Our group also develops unique protocols and techniques for purifying biologically and immunologically active proteins.
Research Professor, Department of Microbiology at NYU Grossman School of Medicine
Professor Emeritus of Microbiology, Department of Microbiology at NYU Grossman School of Medicine
PhD from Loyola University
Fellowship, NYU School of Medicine, Microbiology
Annals of the New York Academy of Sciences. 2013 May; 1287:17-30
Molecular & cellular biology. 1995 Apr; 15(4):2117-24
Biochemistry. 1994 Jun 14; 33(23):7423-9
Journal of molecular biology. 1994 Jan 07; 235(1):221-30
Nature. 1993 Dec 09; 366(6455):585-7
International immunology. 1993 Jun; 5(6):607-14
Journal of biological chemistry. 1992 Jan 15; 267(2):695-8
Proceedings of the National Academy of Sciences of the United States of America (PNAS). 1991 Oct 15; 88(20):9238-42