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Manijeh Mohraz, PhD

Research Associate Professor, Department of Cell Biology

Keywords
structural studies of ion-transporting ATPases
Summary

on-transporting ATPases, or "ion pumps," couple ATP hydrolysis to active ion translocation across membranes, a function essential for cell viability. Although much is understood about ion pumps, details of their transport mechanisms are unknown. Of utmost importance is delineating the three-dimensional structure at high resolution, which would allow integration of the biochemical and kinetic information. We focus on elucidating the three-dimensional structure of Na,K-ATPase, the Na+,K+ pump in the plasma membrane of all animal cells, using electron microscopy of two-dimensional crystals and image reconstruction from the recorded micrographs, complemented with relevant biochemical investigations.

We obtained the three-dimensional structure of Na,K-ATPase at 2.5-nm resolution and mapped the locations of its two subunits. Moreover, results from the combined structural and biochemical studies have implications for the oligomeric form of the functional unit and how ion transport might occur. Based on these findings, we proposed a model for the form and mechanism of the Na+,K+ pump.

Our continuing goals are to extend the structural resolution to 1.0 nm, which would allow identifying structures such as helices and channels, and pursue structural studies on H,K-ATPase, the stomach acid pump.

Phone

212-263-5357

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These focus areas and their associated publications are derived from medical subject headings from PubMed.
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Research Associate Professor, Department of Cell Biology

Instructor-Histology

PhD from University of Michigan

Production of recombinant Na1K-ATPase for structural studies [Meeting Abstract]

Mohraz, M; Morimoto, T

Molecular biology of the cell. 1999 Nov; 10(5):80A-80A

Reconstitution and crystallization of solubilized Na,K-ATPase [Meeting Abstract]

Mohraz, M

Biophysical journal. 1999 Jan; 76(1):A451-A451

Reconstitution and crystallization of solubilized Na,K-ATPase [Meeting Abstract]

Mohraz, M

Molecular biology of the cell. 1997 Nov; 8(5):484-484

MEMBRANE TOPOLOGY OF THE ALPHA SUBUNIT OF NA,K-ATPASE [Meeting Abstract]

MOHRAZ, M; ARYSTARKHOVA, E; SWEADNER, KJ

Biophysical journal. 1993 Feb; 64(2):A331-A331

STRUCTURAL STUDY OF H,K-ATPASE BY ELECTRON-MICROSCOPY AND IMAGE-PROCESSING [Meeting Abstract]

Mohraz, M; Sathe, S; Smith, PR

Biophysical journal. 1990 Feb; 57(2):A357-A357

Structural studies of H,K-ATPase by electron mciroscopy and image reconstructions [Meeting Abstract]

Mohraz, M; Sathe, S; Smith, PR

Electron microscopy : papers presented at the ... International Congress on Electron Microscopy. 1990; 1:94-95