DNA microarrays allow us to follow global cellular responses to various agents. Using UV light as a paradigmatic damaging agent, we determined that cells make following responses: 1. They change signal transduction and transcription regulators, basically stop what they have been doing and respond to UV. 2. Produce cytokines and chemokine to alert the surrounding tissue to the damage. 3. Produce more energy by increasing mitochondrial proteins and suppressing proteins that use energy. 4. Increase the cornified layer that protects the skin. These responses protect not only the cells, but also the underlying tissues of the organism.
In another study, we described the antiviral effects of Interferon-gamma in skin. Specifically, we found that IFNg suppresses cell cycle and DNA replication ? the cell that does not replicate its own DNA will not replicate the viral either; IFNg boosts tight junction proteins, impeding para-cellular viral entry into the organism, and suppresses epidermal differentiation, which inhibits proliferation of papilloma viruses.
We are extending our studies to other agents that affect skin biology, such as proinflammatory cytokines, DNA damaging agents etc. Recently, we have started a program focused on epidermal stem cells and differentiation. Our goal is to describe the global molecular responses of skin to its environment.
Associate Professor, Ronald O. Perelman Department of Dermatology
Associate Professor, Department of Biochemistry and Molecular Pharmacology
PhD from Massachusetts Institute of Technology
Clinical chemistry & laboratory medicine. 2017 Jun 27; 55:e152-e153
American journal of physiology. Heart & circulatory physiology. 2017 Jun 16; 313(3):H631-H640ajpheart.00256.2017
Journal of investigative dermatology. 2017 May 16; 137(9):1868-1877
Science translational medicine. 2017 Jan 4; 9(371):?-?
Cyclic amp, ERK5, and transdifferentiation of cardiac fibroblasts in the pathogenesis of autoimmune congenital heart block [Meeting Abstract]
Arthritis & rheumatology. 2016 Oct; Conference:(American):3179-3181
Transcriptome profile of cells isolated from a CHB heart support an exuberant inflammatory/pro-fibrotic cascade [Meeting Abstract]
Arthritis & rheumatology. 2016 Oct; Conference:(American):3240-3242
Oncogene. 2016 Jun 16; 35(24):3114-3124
Molecular carcinogenesis. 2016 May; 55(5):563-574