Miroslaw K. Gorny

Miroslaw K. Gorny, MD, PhD

Professor, Department of Pathology

Keywords
characterization of HIV-1 proteins by human monoclonal antibodies
Summary

The HIV-1 infection in humans induces a strong cellular and humoral immune response that is thought to partially control the progression of disease. Human monoclonal antibodies (mAbs) reflect the natural immune response and can be useful both for studying the mechanism of humoral immunity and the characterization of the immunogenic regions of viral proteins. Over the last several years, we developed from the lymphocytes of HIV-seropositive subjects more than 60 human mAbs (see Figure) to structural HIV-1 proteins. We identified several types of neutralizing mAbs against the V2, the V3, and the CD4-binding domain (CD4bd) of gpl20 and the ectodomain of gp41. Use of these mAbs showed laboratory strains of HIV-1 to be most sensitive to neutralization by anti-V3 mAbs followed by anti-CD4bd mAbs. The primary isolates were most effectively neutralized by selected mAbs specific for V3 and gp41. Because the enormous variation among HIV-1 isolates allows them to escape immunological control, we generated bispecific Abs, made by fusion of hybridoma producing anti-V3 and anti-CD4bd mAbs, which synergistically enhanced neutralization and broadened the specificity. Besides the research relevance of human mAbs, they may be useful in prophylactic immunotherapy against HIV-1 infection, as several mAbs, including one of our anti-V3 mAbs, proved to be protective against virus in chimpanzee and Hu-PBL-SCID HIV-1 infection models.

 

 

These focus areas and their associated publications are derived from medical subject headings from PubMed.
represents one publication
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Professor, Department of Pathology

PhD from Medical Academy of Wroc?aw

MD from Medical Academy of Wroc?aw

Nanfack, Aubin J; Redd, Andrew D; Bimela, Jude S; Ncham, Genesis; Achem, Emmanuel; Banin, Andrew N; Kirkpatrick, Allison R; Porcella, Stephen F; Agyingi, Lucy A; Meli, Josephine; Colizzi, Vittorio; Nadas, Arthur; Gorny, Miroslaw K; Nyambi, Phillipe N; Quinn, Thomas C; Duerr, Ralf

Journal of clinical microbiology. 2017 Jun 28; 55(9):2785-2800

Monoclonal antibodies specific for the V2, V3, CD4-binding site, and gp41 of HIV-1 mediate phagocytosis in a dose-dependent manner

Musich, T; Li, L; Liu, L; Zolla-Pazner, S; Robert-Guroff, M; Gorny, M K

Journal of virology. 2017 Apr 01; 91(8):e02325-16

Musich, Thomas; Li, Liuzhe; Liu, Lily; Zolla-Pazner, Susan; Robert-Guroff, Marjorie; Gorny, Miroslaw K

Journal of virology. 2017 Mar 29; 91(8):?-?

Balasubramanian, Preetha; Kumar, Rajnish; Williams, Constance; Itri, Vincenza; Wang, Shixia; Lu, Shan; Hessell, Ann J; Haigwood, Nancy L; Sinangil, Faruk; Higgins, Keith W; Liu, Lily; Li, Liuzhe; Nyambi, Phillipe; Gorny, Miroslaw K; Totrov, Maxim; Nadas, Arthur; Kong, Xiang-Peng; Zolla-Pazner, Susan; Hioe, Catarina E

Vaccine. 2017 Feb 6; 35(10):1464-1473

Reed, Joanne H; Gorny, Miroslaw K; Li, Liuzhe; Cardozo, Timothy; Buyon, Jill P; Clancy, Robert M

Journal of autoimmunity. 2017 Jan 22; 79:99-104

Costa, Matthew; Pollara, Justin; Edwards, Regina Whitney; Seaman, Michael; Gorny, Miroslaw K; Montefiori, David; Liao, Hua-Xin; Ferrari, Guido; Lu, Shan; Wang, Shixia

Journal of virology. 2016 Nov 15; 90(22):10362-10378

Hessell, Ann J; McBurney, Sean; Pandey, Shilpi; Sutton, William; Liu, Lily; Li, Liuzhe; Totrov, Maxim; Zolla-Pazner, Susan; Haigwood, Nancy L; Gorny, Miroslaw K

Vaccine. 2016 May 23; 34(24):2713-2721

McFarren, Alicia; Lopez, Lillie; Williams, Dionna W; Veenstra, Mike; Bryan, Ruth A; Goldsmith, Aliza; Morgenstern, Alfred; Bruchertseifer, Frank; Zolla-Pazner, Susan; Gorny, Miroslaw K; Eugenin, Eliseo A; Berman, Joan W; Dadachova, Ekaterina

AIDS. 2016 Feb 20; 30(4):563-572