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Miroslaw K. Gorny

Miroslaw K. Gorny, MD, PhD

Professor, Department of Pathology

Keywords
characterization of HIV-1 proteins by human monoclonal antibodies
Summary

The HIV-1 infection in humans induces a strong cellular and humoral immune response that is thought to partially control the progression of disease. Human monoclonal antibodies (mAbs) reflect the natural immune response and can be useful both for studying the mechanism of humoral immunity and the characterization of the immunogenic regions of viral proteins. Over the last several years, we developed from the lymphocytes of HIV-seropositive subjects more than 60 human mAbs (see Figure) to structural HIV-1 proteins. We identified several types of neutralizing mAbs against the V2, the V3, and the CD4-binding domain (CD4bd) of gpl20 and the ectodomain of gp41. Use of these mAbs showed laboratory strains of HIV-1 to be most sensitive to neutralization by anti-V3 mAbs followed by anti-CD4bd mAbs. The primary isolates were most effectively neutralized by selected mAbs specific for V3 and gp41. Because the enormous variation among HIV-1 isolates allows them to escape immunological control, we generated bispecific Abs, made by fusion of hybridoma producing anti-V3 and anti-CD4bd mAbs, which synergistically enhanced neutralization and broadened the specificity. Besides the research relevance of human mAbs, they may be useful in prophylactic immunotherapy against HIV-1 infection, as several mAbs, including one of our anti-V3 mAbs, proved to be protective against virus in chimpanzee and Hu-PBL-SCID HIV-1 infection models.

 

 

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Professor, Department of Pathology

PhD from Medical Academy of Wroc?aw

MD from Medical Academy of Wroc?aw

Kaku, Yu; Kuwata, Takeo; Gorny, Miroslaw K; Matsushita, Shuzo

Japanese journal of infectious diseases. 2020 Jan 31;

Duerr, Ralf; Gorny, Miroslaw K

Animals : an open access journal from MDPI. 2019 Aug 03; 9(8):

Banin, Andrew N; Tuen, Michael; Bimela, Jude S; Tongo, Marcel; Zappile, Paul; Khodadadi-Jamayran, Alireza; Nanfack, Aubin J; Okonko, Iheanyi O; Meli, Josephine; Wang, Xiaohong; Mbanya, Dora; Ngogang, Jeanne; Gorny, Miroslaw K; Heguy, Adriana; Fokunang, Charles; Duerr, Ralf

Journal of the International AIDS Society. 2019 Jul; 22(7):e25362

Liu, Lily; Li, Liuzhe; Nanfack, Aubin; Mayr, Luzia M; Soni, Sonal; Kohutnicki, Adam; Agyingi, Lucy; Wang, Xiao-Hong; Tuen, Michael; Shao, Yongzhao; Totrov, Maxim; Zolla-Pazner, Susan; Kong, Xian-Peng; Duerr, Ralf; Gorny, Miroslaw K

Virology. 2019 Jan 14; 529:57-64

Tuen, Michael; Bimela, Jude S; Banin, Andrew N; Ding, Shilei; Harkins, Gordon W; Weiss, Svenja; Itri, Vincenza; Durham, Allison R; Porcella, Stephen F; Soni, Sonal; Mayr, Luzia; Meli, Josephine; Torimiro, Judith N; Tongo, Marcel; Wang, Xiaohong; Kong, Xiang-Peng; Nádas, Arthur; Kaufmann, Daniel E; Brumme, Zabrina L; Nanfack, Aubin J; Quinn, Thomas C; Zolla-Pazner, Susan; Redd, Andrew D; Finzi, Andrés; Gorny, Miroslaw K; Nyambi, Phillipe N; Duerr, Ralf

Frontiers in immunology. 2019; 10:1062

Chan, Kun-Wei; Pan, Ruimin; Costa, Matthew; Gorny, Miroslaw K; Wang, Shixia; Lu, Shan; Kong, Xiang-Peng

Journal of virology. 2018 09 15; 92(18):

Cheng, Hao D; Grimm, Sebastian K; Gilman, Morgan Sa; Gwom, Luc Christian; Sok, Devin; Sundling, Christopher; Donofrio, Gina; Hedestam, Gunilla B Karlsson; Bonsignori, Mattia; Haynes, Barton F; Lahey, Timothy P; Maro, Isaac; von Reyn, C Fordham; Gorny, Miroslaw K; Zolla-Pazner, Susan; Walker, Bruce D; Alter, Galit; Burton, Dennis R; Robb, Merlin L; Krebs, Shelly J; Seaman, Michael S; Bailey-Kellogg, Chris; Ackerman, Margaret E

JCI insight. 2018 03 08; 3(5):