In most organisms, primordial germ cells (PGCs) are set-aside early during embryogenesis. Subsequently, PGCs migrate through the embryo, associate with somatic gonadal cells and form the gonad. While PGCs seem highly specialized, their products are the ultimate stem cells that generate a complete organism generation after generation. Our long-term goal is to functionally dissect the germ cell life cycle in Drosophila. Projects in the lab focus on three areas. 1) PGC specification. Early Drosophila germ cells form in a specialized germplasm and upon formation are transcriptionally silent. We are interested how RNAs localized to germ plasm are regulated and contribute to germ cell formation and fate, how gene expression is regulated during germ cell development and how the reproductive success of individual PGCs is determined. 2) PGC migration. Taking advantage of in vitro migration assays and live imaging, we study molecular and cellular aspects of germ cell migration to understand how the migratory program is established, how phospholipid signals contribute guidance cues to PGCs and how PGCs cease migration once they reach the somatic gonad. 3) Germ cell genome protection. Germ cells give rise to a new generation, thus integrity of the germ cell genome has to be protected while at the same time there has to be room for improvement via mutation and recombination. We study how host mechanisms evolved to protect the genome against transposable elements and how other intruders such as bacteria influence germ line function and reproductive success.
Laura and Isaac Perlmutter Professor of Cell Biology, Department of Cell Biology
Chair, Department of Cell Biology
Director, Skirball Institute of Biomolecular Medicine
PhD from University Tubingen
Nature protocols. 2017 Jul; 12(7):1326-1348
eLife. 2017 Jan 24; 6:?-?
Current biology. CB. 2017 Jan 23; 27(2):291-297
Developmental cell. 2016 Dec 05; 39(5):560-571
Current opinion in cell biology. 2016 Oct; 42:128-137
Science. 2016 May 20; 352(6288):899-901
Cell stem cell. 2016 Feb 04; 18(2):276-290
Current topics in developmental biology. 2016; 116:679-707