Warren R. Jelinek

Warren R. Jelinek, PhD

Professor, Department of Biochemistry and Molecular Pharmacology

Keywords
terminal differentiation in murine erythroleukemia cells
Summary

We seek to identify molecules that act during the pre-commitment period to cause the commitment event of terminal differentiation in murine erythroleukemia cells (MELC). We mutagenized MELC by retroviral insertion and isolated mutants that fail to commit to the terminal erythrodifferentiation program. Repeatedly, and independently, one particular genomic locus was the insertional target in fail-to-commit mutants. We now seek to identify the gene associated with this locus. We identified a novel mammalian protein kinase, which we termed STPK13, whose mRNA declines dramatically during the MELC pre-commitment period. Further investigation demonstrated that the mRNA also fluctuates in abundance during the normal cell cycle. Homologous genes, termed polo and cdc5 have recently been isolated from Drosophila and Saccharomyces cerevisiae, respectively. Analyses of mutants of these genes have revealed a requirement during mitosis for the encoded protein kinase. The cell cycle-associated mRNA fluctuations we observed in mouse cells is conserved in S. cerevisiae and thus has been maintained during at least five hundred million years of evolution; it is expected to be a fundamental mechanism of regulation of the protein kinase. Our recent observations suggest that cell cycle-associated regulation of pre-mRNA splicing plays a central role in the cell cycle-associated fluctuation of the abundance of this mRNA. The decline in STPK13 mRNA abundance during the MELC pre-commitment period is expected to indicate an anticipation by these cells of a halt in cell division associated with the commitment event. We recently identified a protein target of association of the STPK13 protein kinase. It is a highly conserved RNA helicase which also shows cell cycle regulation. Both proteins are potentially good candidates as antimitotic targets.

Phone

212-263-5937

Academic office

277 East 30th Street, Verizon Bldg.

7, 726

New York, NY 10016

Is this your profile?
These focus areas and their associated publications are derived from medical subject headings from PubMed.
represents one publication
Loading...

Professor, Department of Biochemistry and Molecular Pharmacology

Chair - IBC Committee

PhD from University of Pennsylvania

Johnson EM; Jelinek WR

Proceedings of the National Academy of Sciences of the United States of America (PNAS). 1986 Jul; 83(13):4660-4664

Kaplan G; Jelinek WR; Bachvarova R

Developmental biology (Orlando). 1985 May; 109(1):15-24

DiGiovanni L; Haynes SR; Misra R; Jelinek WR

Proceedings of the National Academy of Sciences of the United States of America (PNAS). 1983 Nov; 80(21):6533-6537

ANALYSIS OF RNA SPECIES IN MOUSE OOCYTES BY NORTHERN BLOTTING - ACTIN MESSENGER-RNA, U1-RNA, AND ALU SEQUENCES [Meeting Abstract]

BACHVAROVA, R; KAPLAN, G; JELINEK, WR

Journal of cell biology. 1983; 97(5):A20-A20

Jelinek WR; Haynes SR

Cold Spring Harbor symposia on quantitative biology. 1983; 47 Pt 2:1123-1130