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The structure and retrotransposition mechanism of LTR-retrotransposons in the asexual yeast 
Candida albicans.


 

Affinity proteomics catches elusive jumping genes in the act. LINE-1s are active human DNA parasites that are agents of genome dynamics in evolution and disease. These streamlined elements require host factors to complete their life cycles, whereas hosts have developed mechanisms to combat retrotransposition's mutagenic effects. As such, endo- genous L1 expression levels are extremely low, creating a roadblock for detailed interactomic analyses. Here, we describe a system to express and purify highly active L1 RNP complexes from human suspension cell culture and characterize the copurified proteome. More...