FACSAria IIu SORP | NYU Langone Health

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Cytometry & Cell Sorting Laboratory Instruments & Services FACSAria IIu SORP

FACSAria IIu SORP

The expert staff at NYU Langone’s Cytometry and Cell Sorting Laboratory can assist you in using a FACSAria IIu SORP cell sorter. Reserve this cell sorter using iLab.

The FACSAria™ IIu SORP Cell Sorter

FACSAria IIu SORP Location

NYC Health + Hospitals/Bellevue
462 First Avenue
C&D Building, Room CD645
Phone: 212-263-5063

FACSAria IIu SORP Optics

5 lasers and 17 colors (see configuration)

FACSAria IIu SORP Biosafety Level

FACSAria IIu SORP Pricing

  • $144 per hour: Perlmutter Cancer Center users
  • $155 per hour: non–Perlmutter Cancer Center users
  • $206 per hour: external academic users
  • $258 per hour: external industry users

FACSAria IIu SORP Sample Preparation

  • Acceptable sample tubes are polypropylene 12 × 75 mm tubes, 15-mL tubes, bullet tubes, or 1.5-mL microcentrifuge tubes.
  • Concentration should be 10–20 × 106 cells/mL (allows for ideal pressure and best use of time).
  • Always bring extra media to dilute if necessary.
  • Filter all samples as close to sorting time as possible.

FACSAria IIu SORP Sample Collection

Collection tubes should be coated with appropriate media, such as FBS:

  • 4 5-mL (12 × 75 mm) tubes
  • 2 15-mL tubes
  • 4 1.5-mL centrifuge tubes
  • well plates (up to 384 wells)
  • 4 “bullet” tubes (1-mL tubes)
  • 4 polymerase chain reaction (PCR) tubes
  • slides and cover slips

FACSAria IIu SORP Nozzle Options

70 µm

  • This nozzle can sort 9 × 107 total cells per hour; collection volume is ~106 cells in 1 mL.
  • It requires higher pressure (70 psi) and is not ideal for larger or fragile cells.

100 µm

  • This nozzle can sort 5.4 × 107 cells per hour; collection volume is ~4 × 105 cells in 1 mL.
  • It is best for large or delicate cells because it uses lower pressure (20 psi).
  • Examples include stem cells, fibroblasts, neurons, and larger cancer cells.
  • Thirty minutes’ extra setup time is required.

FACSAria IIu SORP Configuration

Review the configuration of the FACSAria IIu SORP below, or access a printable document

Aria II (5 laser, 18 parameters) 

Configuration of the FACSAria™ IIu SORP Cell Sorter

Aria II Filter Setup

FACSAria™ IIu SORP Cell Sorter Filter Setup