MoFlo XDP | NYU Langone Health

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Cytometry & Cell Sorting Laboratory Instruments & Services MoFlo XDP

MoFlo XDP

The expert staff at NYU Langone’s Cytometry and Cell Sorting Laboratory can assist you in using a MoFlo XDP cell sorter. Reserve this instrument using iLab.

The MoFlo™ XDP Cell Sorter

MoFlo XDP Location

NYU Langone 
540 First Avenue
Skirball Institute of Biomolecular Medicine
3rd Floor, Lab 9
Phone: 212-263-5907

MoFlo XDP Optics

4 lasers and 11 colors (see configuration)

MoFlo XDP Biosafety Level

MoFlo XDP Pricing

  • $144 per hour: Perlmutter Cancer Center users
  • $155 per hour: non–Perlmutter Cancer Center users
  • $206 per hour: external academic users
  • $258 per hour: external industry users

MoFlo XDP Sample Preparation

  • Use only 12 × 75 mm sample tubes (polypropylene is recommended).
  • Concentration should be 10–20 × 106 cells/mL (allows for ideal pressure and best use of time).
  • Always bring extra media to dilute if necessary.
  • Filter all samples as close to sorting time as possible.

MoFlo XDP Sample Collection

Collection tubes should be coated with appropriate media (such as fetal bovine serum, or FBS):

  • 4 5-mL (12 × 75 mm) tubes
  • 4 1.5-mL microcentrifuge tubes
  • slides and cover slips
  • well plates (up to 384 wells each)

MoFlo XDP Nozzle Options

70 µm

  • This nozzle can sort 8.8 × 107 total cells per hour; collection volume is ~106 cells in 1 mL.
  • It requires higher pressure (60 psi) and is not ideal for larger or fragile cells.

100 µm

  • This nozzle can sort 4.5 × 107 cells per hour; collection volume is ~4 × 105 cells in 1 mL.
  • It is best for large or delicate cells because it uses lower pressure (30 psi).
  • Examples include stem cells, fibroblasts, neurons, and larger cancer cells.
  • It requires 30 minutes’ extra setup time.

MoFlo XDP Configuration

Review the configuration of the MoFlo XDP below, or access a printable document

Configuration of the MoFlo™ XDP Cell Sorter