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Ultrasound and Magnetic Resonance Microimaging of Transgenic Mice

Extensive genetic information and the expanding number of techniques available to manipulate the genome of the mouse have led to its widespread use in studies of development and to model human diseases. In this rapid proliferation of methods to genetically engineer mice, in vivo technologies to analyze structure and function in the mouse have not kept pace. The results of transgenic and gene targeting experiments, for the most part, are analyzed using histological methods which are static and two-dimensional, making it difficult to understand the underlying developmental and disease processes which are three-dimensional (3D) and dynamic, evolving over variable time periods. Noninvasive imaging methods such as ultrasound and magnetic resonance imaging (MRI) are essential clinical tools for assessing fetal health, and for diagnosing and staging many human diseases including cancer, heart disease and many neuro-degenerative disorders. The Turnbull laboratory is developing a combination of ultrasound and MRI microimaging approaches with sufficient resolution and sensitivity to provide noninvasive structural, functional and molecular data on developmental and disease processes in normal and genetically-engineered mice.



Sagittal in utero UBM image of an E11.5 mouse embryo.

3D MRI of the vasculature of an E11.5 mouse embryo.

3D MRI of the head and brain of a P5 mouse neonate.

Three-dimensional in vivo MRI with self-gating and image coregistration in the mouse. Magn Reson Med 61: 1148-57 by Nieman BJ, Szulc KU, Turnbull DH (2009).


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