Klein Lab

Klein_photo

Catherine Klein, Ph.D.

Assistant Professor, Department of Environmental Medicine

Contact Info:

Address: 57 Old Forge Road, Tuxedo, NY 10987
Phone: 845-731-3510
Email: Catherine.Klein@nyumc.org

 

 

Key Interests

Diethylstilbestrol, DNA methylation, epigenesis, estrogens, transgene, xenoestrogens

Biographic Details:

Graduate Education: 1988 — Ph.D. from New York University

Research Interests:

With molecular biologic and molecular cytogenetic techniques, we study the mutagenic and epigenetic mechanisms that silence transgene expression in a unique set of mammalian cell lines. The G12 and G10 cell lines each contain a bacterial xanthine-guanine phosphoribosyl transferase (gpt) gene target that has been stably inserted into different chromosomal genome locations. These cell lines exhibit differing propensities for mutagenesis and deletions by oxidative carcinogens and clastogens, including x-rays and anticancer therapeutics, e.g., bleomycin and amsacrine, with the G10 cells showing the most sensitivity to these compounds. In contrast to clastogen mutagenesis, some traditionally nonmutagenic carcinogens such as nickel, diethylstilbestrol (DES), and 17b-estradiol have been found to inactivate gpt expression solely or predominantly in the G12 but not G10 cells. Epigenetic silencing of the gpt transgene by DNA condensation and excess methylation has been identified as the mechanism for trangene silencing in the nickel-induced G12 mutants. Preliminary data suggest that this is also true for many DES-induced mutants. Similar studies are being performed for other natural and synthetic estrogens, as well as for putative xenoestrogens such as organic pesticides.

Selected Publications:

A Historical Overview of Cell-Based Assays for Detecting Epigenetic Effects. 
Klein, C.
2012 SEP;53 1 1:S41-S41, Environmental & molecular mutagenesis
— id: 177763, year: 2012, vol: 53 1 1, page: S41, stat: Journal Article,

Impairment of antioxidant defenses as a contributor to arsenite-induced cell transformation
. Wu, Jing; Sowinska, Anna; Huang, Xi; Klein, Catherine B; Pelle, Edward; Frenkel, Krystyna
2012 Oct;25(5):927-937, Biometals
— id: 177140, year: 2012, vol: 25, page: 927, stat: Journal Article,

Genes expressed in Atoh1 neuronal lineages arising from the r1/isthmus rhombic lip. 
Machold, R; Klein, C; Fishell, G
2011 Jun-Jul;11(5-6):349-359, Gene expression patterns
— id: 132573, year: 2011, vol: 11, page: 349, stat: Journal Article,

Genetic and epigenetic effects of environmental arsenicals. 
Rossman, Toby G; Klein, Catherine B
2011 Nov 1;3(11):1135-1141, Metallomics : integrated biometal science
— id: 140528, year: 2011, vol: 3, page: 1135, stat: Journal Article,

Caffeic acid phenethyl ester (CAPE), derived from a honeybee product propolis, exhibits a diversity of anti-tumor effects in pre-clinical models of human breast cancer. 
Wu, Jing; Omene, Coral; Karkoszka, Jerzy; Bosland, Maarten; Eckard, Jonathan; Klein, Catherine B; Frenkel, Krystyna
2011 Sep 1;308(1):43-53, Cancer letters
— id: 134448, year: 2011, vol: 308, page: 43, stat: Journal Article,

See All Publications