Wirgin Lab

Wirgin_photo

Isaac Wirgin, Ph.D.

Associate Professor, Department of Environmental Medicine

Contact Info:

Address: 57 Old Forge Road, Tuxedo, NY 10987
Phone: 845-731-3548
Email: Isaac.Wirgin@nyumc.org

 

 

Key Interests:

chemical carcinogenesis, gene regulation, genetic variability, Hudson River pollution, mitochondrial DNA, oncogene activation

Biographic Details

Graduate Education: 1987 — City University of New York, New York, NY

Research Interests:

The Atlantic tomcod, Microgadus tomcod, from the Hudson River provides a unique opportunity to study the molecular aspects of genetic susceptibility to neoplasia in an outbred model organism that 1) exhibits an unusually high prevalence of hepatocellular carcinomas (more than 90% in 2-yr-old fish), 2) is exposed to high levels of environmentally borne chemical carcinogens, and 3) can be used to investigate the effects of controlled laboratory model-chemical exposures on sensitive early-life stages.

We examined the effects of the interactions of genetic variation and exposure to high levels of Hudson River-borne xenobiotics on the structure and expression of carcinogenically relevant genes, such as K-ras, aromatic hydrocarbonreceptor (Ahr) and cytochrome P4501A (CYP1A1). We found activated K-ras in a high percentage of tomcod liver-tumor DNAs, a transcribed genetic polymorphism in the 3' untranslated region of CYP1A1 in Hudson River tomcod that is not observed in cancer-free populations of tomcod.

Currently, we study the functional significance of genetic variation in this model relevant to: 1) the role of K-ras oncogene activation in early neoplastic events and hepatocarcinogenesis progression in tomcod livers, 2) the effects of environmentally relevant polychlorobiphenyl (PCB) promotion on clonal expansion of initiated cells, and 3) the molecular mechanisms of CYP1A1 gene regulation in environmentally exposed tomcod from the Hudson River. We also explore the effects of inter-specific and inter-individual differences in Ahr gene expression levels on CYP1A1 gene expression and transcription inhibition in halogenated aromatic hydrocarbon-exposed fish.

Selected Publications:

Selected polymorphisms in sex hormone-related genes, circulating sex hormones and risk of endometrial cancer. Lundin, Eva; Wirgin, Isaac; Lukanova, Annekatrin; Afanasyeva, Yelena; Krogh, Vittorio; Axelsson, Tomas; Hemminki, Kari; Clendenen, Tess V; Arslan, Alan A; Ohlson, Nina; Sieri, Sabina; Roy, Nirmal; Koenig, Karen L; Idahl, Annika; Berrino, Franco; Toniolo, Paolo; Hallmans, Goran; Forsti, Asta; Muti, Paola; Lenner, Per; Shore, Roy E; Zeleniuch-Jacquotte, Anne. 2012 Oct;36(5):445-452, Cancer Epidemiology. — id: 178879, year: 2012, vol: 36, page: 445, stat: Journal Article,

Mixed-stock analysis of wintertime aggregations of striped bass along the Mid-Atlantic coast. Waldman, J.; Maceda, L.; Wirgin, I.. 2012 FEB;28(1):1-6, Journal of applied ichthyology. — id: 157625, year: 2012, vol: 28, page: 1, stat: Journal Article,

Characterization and expression of cytochrome P4501A in Atlantic sturgeon and shortnose sturgeon experimentally exposed to coplanar PCB 126 and TCDD. Roy NK; Walker N; Chambers RC; Wirgin I. 2011 Mar 30;104(1-2):23-31, Aquatic toxicology. — id: 132243, year: 2011, vol: 104, page: 23, stat: Journal Article,

Mechanistic basis of resistance to PCBs in Atlantic tomcod from the Hudson River
. Wirgin, Isaac; Roy, Nirmal K; Loftus, Matthew; Chambers, R Christopher; Franks, Diana G; Hahn, Mark E. 2011 Mar 11;331(6022):1322-1325, Science. — id: 127231, year: 2011, vol: 331, page: 1322, stat: Journal Article,

See All Publications