Catherine G. Klein

Catherine G. Klein, PhD

Assistant Professor, Department of Environmental Medicine

Keywords
mechanisms of gene silencing, epigenetics, gene methylation, environmental toxicants, metals
Summary

With molecular biologic and molecular cytogenetic techniques, we study the mutagenic and epigenetic mechanisms that silence genes, including transgene expression in a unique set of mammalian cell lines. The G12 and G10 cell lines each contain a bacterial xanthine-guanine phosphoribosyl transferase (gpt) gene target that has been stably inserted into different chromosomal genome locations. These cell lines exhibit differing propensities for mutagenesis, deletions and epigenetic gene silencing by various oxidative carcinogens and clastogens, including x-rays, anticancer therapeutics, metals and traditionally nonmutagenic carcinogens such as diethylstilbestrol (DES). The assay offers the ability to analyze the epigenetic potential of other putative xenoestrogens or environmental toxicants, such as organic pesticides, environmental mixtures and nanoparticles. Using this assay system, the epigenetic silencing of the gpt transgene by DNA condensation, excess methylation and histone modifications were identified as important mechanisms for trangene silencing in carcinogenic nickel-induced G12 mutants. Epigenome arrays add complementary information about other genes and pathways that can be altered by a variety of environmental exposures.

Phone

845-731-3510

Academic office

57 Old Forge Rd.

Second Floor

Tuxedo, NY 10987

Lab Website
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These focus areas and their associated publications are derived from medical subject headings from PubMed.
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Assistant Professor, Department of Environmental Medicine

Dir Environmental Health Science Graduate Pgm

Dir Mole Cell and Anal Svcs Fac Core NYU NIEHS Ctr

Mgr Anal Svs Unit, Exper Anml Exp Core NYUCC

PhD from New York University

Morgan, Helen; Zeller, John; Hughes, David T; Dooley-Hash, Suzanne; Klein, Katherine; Caty, Rachel; Santen, Sally

Surgical & radiologic anatomy : SRA. 2016 Jun 15; 39(1):95-101

Lauterstein, Dana E; Tijerina, Pamella B; Corbett, Kevin; Akgol Oksuz, Betul; Shen, Steven S; Gordon, Terry; Klein, Catherine B; Zelikoff, Judith T

International journal of environmental research & public health. 2016 Apr 12; 13(4):417-417

Mauro, Maurizio; Caradonna, Fabio; Klein, Catherine B

Environmental & molecular mutagenesis. 2016 Mar; 57(2):137-150

E-Cigarette Aerosols Induce Neurobiological and Neurobehavioral Alterations in Mice Exposed during Early Life [Meeting Abstract]

Lauterstein, D. E.; Tijerina, P. B.; Corbett, K.; Gordon, T.; Aschner, M.; Parmalee, N.; Conrad, K.; Allen, J.; Cory-Slechta, D.; Zelikoff, J. T.; Klein, C. B.

Environmental & molecular mutagenesis. 2016 Sep; 57:S81-S81

Epigenetic assays: Updates, benefits, and limitations [Meeting Abstract]

Klein, C B

Environmental & molecular mutagenesis. 2014 Sep; 55:S27-S27

Current and future prospectives for jobs in science [Meeting Abstract]

Klein, C B

Environmental & molecular mutagenesis. 2014 Sep; 55:S19-S19

Klein, Catherine; Costa, Max

Handbook on the toxicology of metals. Amsterdam : Academic Press, 2014. 4th ed.. p.1091-1111. (2061012)

Wilson, Thomas E; Demarini, David M; Dertinger, Stephen D; Engelward, Bevin P; Hanawalt, Philip C; Macgregor, James T; Smith-Roe, Stephanie L; Witt, Kristine L; Yauk, Carole L; Ljungman, Mats; Schwartz, Jeffrey L; Klein, Catherine B

Environmental & molecular mutagenesis. 2013 Apr; 54(3):153-157