Eli Rothenberg, PhD

Our research program focuses on understanding how cells maintain genome integrity and how dysfunctions in these processes contribute to cancer and other diseases. To investigate these mechanisms at the molecular level, we use specialized single-molecule microscopy techniques and innovative assays. Our multidisciplinary approach combines single-molecule fluorescence microscopy, computational tools, and advanced methods in biochemistry, molecular biology, cell biology, and genetic engineering.

These tools allow us to uncover the unique properties of DNA damage response and replication pathways in cancer cells, which are critical for controlling tumor growth and therapeutic outcomes. We also study the role of non-canonical DNA structures, such as G-quadruplexes (G4) and R-loops, and how their faulty metabolism influences mutagenesis and therapy resistance. Our research further explores how mutations in DNA replication and repair genes, like BRCA, affect repair processes and lesion toxicity.

A major part of our work involves advancing pharmacological studies by providing insights into the mechanisms of emerging cancer drugs and understanding how cancer cells respond to chemotherapies and radiation treatments. We are also developing new biomarkers for targeted therapies and screening applications, helping to address mutagenic repair and therapy resistance.

In parallel, the methods we develop are applied to studying molecular mechanisms in other systems, including neuronal development, cardiac cell biology, viral infections, membrane signaling, mitochondrial biology, and cellular metabolism.