Hannah L. Klein

Hannah L. Klein

Professor, Department of Biochemistry and Molecular Pharmacology

Professor, Department of Medicine

Professor, Department of Pathology

Keywords
pathways of DNA damage avoidance, DNA repair and recombination, genomic instability, DNA damage checkpoints, cancer
Summary

While damage to DNA occurs frequently, it is increased in many tumor cells. Therefore, identifying pathways involved in genome maintenance is important for understanding how DNA damage can arise, its consequences, and how it can be prevented. We have two areas of investigation through which we study DNA repair pathways and how the repair of DNA damage is regulated.

We focus mainly on the regulation of DNA motor proteins. These proteins move along the DNA duplex and remove molecules that form obstacles to sensing DNA damage and repairing it through homologous recombination (HR). We have found that modification of these motor proteins through phosphorylation is required for regulating their action and limiting the occurrence of HR to the right situation. Too much HR, or too little, can destabilize the genome. Therefore, regulation of these key proteins is essential for maintaining both the right balance of HR and genome stability.

The second area of our research centers around the occurrence of aberrant replication intermediates in DNA. Although DNA is composed primarily of deoxyribonucleotide residues, with significant frequency ribonucleotide residues are misincorporated into DNA instead. An enzyme called RNase H2 recognizes and removes ribonucleotides from DNA, but when this process is defective DNA damage increases and several genome errors occur, including mutations, chromosome loss, and increased HR. We have identified some of the proteins that protect the genome against damage from ribonucleotides in DNA, and we continue to study the mechanisms by which they maintain the genome.

Phone

212-263-5778

Fax

212-263-9484

Academic office

NYU Langone Medical Center, 550 First Avenue

Smilow 201

New York, NY 10016

Lab Website
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Professor, Department of Biochemistry and Molecular Pharmacology

Professor, Department of Medicine

Professor, Department of Pathology

Co-Course Director, Current Topics in Genome Integrity

Course Director, Genetics

Vice Chair, Department of Biochemistry and Molecular Pharmacology

Potenski, Catherine J; Niu, Hengyao; Sung, Patrick; Klein, Hannah L

Nature. 2014 Jul 10; 511(7508):251-254

Niu, Hengyao; Potenski, Catherine J; Epshtein, Anastasiya; Sung, Patrick; Klein, Hannah L

Cell cycle. 2016; 15(3):331-336

Potenski, Catherine J; Klein, Hannah L

Nucleic acids research. 2014; 42(16):10226-10234

Shah, Parisha P; Zheng, Xiuzhong; Epshtein, Anastasiya; Carey, Jeffrey N; Bishop, Douglas K; Klein, Hannah L

Molecular cell. 2010 Sep 24; 39(6):862-872

Klein, Hannah L

DNA repair (Amsterdam). 2017 Jun 9; 56:26-32

Niu, Hengyao; Klein, Hannah L

FEMS yeast research. 2017 Mar 01; 17(2):

Jinks-Robertson, Sue; Klein, Hannah L

Nature structural & molecular biology. 2015 Mar; 22(3):176-178