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Hannah L. Klein

Hannah L. Klein, PhD

Professor, Department of Biochemistry and Molecular Pharmacology

Professor, Department of Medicine

Professor, Department of Pathology

pathways of DNA damage avoidance, DNA repair and recombination, genomic instability, DNA damage checkpoints, cancer

While damage to DNA occurs frequently, it is increased in many tumor cells. Therefore, identifying pathways involved in genome maintenance is important for understanding how DNA damage can arise, its consequences, and how it can be prevented. We have two areas of investigation through which we study DNA repair pathways and how the repair of DNA damage is regulated.

We focus mainly on the regulation of DNA motor proteins. These proteins move along the DNA duplex and remove molecules that form obstacles to sensing DNA damage and repairing it through homologous recombination (HR). We have found that modification of these motor proteins through phosphorylation is required for regulating their action and limiting the occurrence of HR to the right situation. Too much HR, or too little, can destabilize the genome. Therefore, regulation of these key proteins is essential for maintaining both the right balance of HR and genome stability.

The second area of our research centers around the occurrence of aberrant replication intermediates in DNA. Although DNA is composed primarily of deoxyribonucleotide residues, with significant frequency ribonucleotide residues are misincorporated into DNA instead. An enzyme called RNase H2 recognizes and removes ribonucleotides from DNA, but when this process is defective DNA damage increases and several genome errors occur, including mutations, chromosome loss, and increased HR. We have identified some of the proteins that protect the genome against damage from ribonucleotides in DNA, and we continue to study the mechanisms by which they maintain the genome.





Academic office

The Alexandria Center of Life, 450 East 29th Street

East Tower, 901

New York, NY 10016

Lab Website
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These focus areas and their associated publications are derived from medical subject headings from PubMed.
represents one publication
*Due to PubMed processing times, the most recent publications may not be reflected in the timeline.

PhD from University of Washington

Potenski, Catherine J; Niu, Hengyao; Sung, Patrick; Klein, Hannah L

Nature. 2014 Jul 10; 511(7508):251-4

Niu, Hengyao; Potenski, Catherine J; Epshtein, Anastasiya; Sung, Patrick; Klein, Hannah L

Cell cycle. 2016 May; 15(3):331-6

Potenski, Catherine J; Klein, Hannah L

Nucleic acids research. 2014 Nov; 42(16):10226-34

Shah, Parisha P; Zheng, Xiuzhong; Epshtein, Anastasiya; Carey, Jeffrey N; Bishop, Douglas K; Klein, Hannah L

Molecular cell. 2010 Sep 24; 39(6):862-72

Sui, Yang; Epstein, Anastasiya; Dominska, Margaret; Zheng, Dao-Qiong; Petes, Thomas D; Klein, Hannah L

Nucleic acids research. 2022 Jun 24; 50(12):6890-902

Epshtein, Anastasiya; Symington, Lorraine S; Klein, Hannah L

Methods in molecular biology. 2021; 2153:193-200

Klein, Hannah L

Proceedings of the National Academy of Sciences of the United States of America (PNAS). 2020 May 12; 117(19):10108-10110