Mamta V. Tahiliani, PhD

Skirball Institute

Assistant Professor, Department of Biochemistry and Molecular Pharmacology

Keywords
cancer, developmental genetics, genome integrity, genomics, immunology, metabolism, pharmacology, stem cell biology
Summary

5-methylcytosine is a minor base in mammalian DNA, which plays a disproportionately critical role in development. DNA methylation is very dynamic during embryogenesis and is vital for parental imprinting, X inactivation, silencing of endogenous retroviruses as well as the regulation of genomic stability. De novo methylation and demethylation also occur in somatic cells during differentiation, tumorigenesis and aging. In contrast to the established relation between DNA methylation, DNA methyltransferases and gene silencing, the enzymes and processes regulating DNA demethylation are not well understood. We have recently discovered that TET1 catalyzes the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). We also found that hmC can be detected in the genome of mouse ES cells, and that both TET1 levels and hmC levels decline when ES cells are differentiated. These results suggest that hmC is a normal constituent of mammalian DNA, and identify TET1 as an enzyme with a potential role in epigenetic regulation through modification of 5mC. This study described a new class of enzymes that catalyze a new modification of DNA and alters our perception of how DNA methylation status may be regulated in cells. The description of the regulated conversion of 5mC to hmC raises an enormous number of new questions that are of pressing importance. The goal of my laboratory is to integrate hmC into known pathways of 5mC metabolism and to determine how hmC exerts its influence on the genome with the ultimate goal of understanding the role that 5mC and hmC play in genomic stability.

Phone

212-263-2854

Academic office

540 First Avenue, Skirball Institute

4, 4

New York, NY 10016

Lab Website
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Assistant Professor, Department of Biochemistry and Molecular Pharmacology

PhD from Harvard University

Li, Cheng; Lan, Yahui; Schwartz-Orbach, Lianna; Korol, Evgenia; Tahiliani, Mamta; Evans, Todd; Goll, Mary G

Cell reports. 2015 Aug 18; 12(7):1133-1143

Chavez, Lukas; Huang, Yun; Luong, Khai; Agarwal, Suneet; Iyer, Lakshminarayan M; Pastor, William A; Hench, Virginia K; Frazier-Bowers, Sylvia A; Korol, Evgenia; Liu, Shuo; Tahiliani, Mamta; Wang, Yinsheng; Clark, Tyson A; Korlach, Jonas; Pukkila, Patricia J; Aravind, L; Rao, Anjana

Proceedings of the National Academy of Sciences of the United States of America (PNAS). 2014 Dec 02; 111(48):E5149-E5158

Kriaucionis, Skirmantas; Tahiliani, Mamta

Cold Spring Harbor perspectives in biology. 2014 Oct 01; 6(10):a018630-a018630a018630

Pastor, William A; Pape, Utz J; Huang, Yun; Henderson, Hope R; Lister, Ryan; Ko, Myunggon; McLoughlin, Erin M; Brudno, Yevgeny; Mahapatra, Sahasransu; Kapranov, Philipp; Tahiliani, Mamta; Daley, George Q; Liu, X Shirley; Ecker, Joseph R; Milos, Patrice M; Agarwal, Suneet; Rao, Anjana

Nature. 2011 May 19; 473(7347):394-397

Koh, Kian Peng; Yabuuchi, Akiko; Rao, Sridhar; Huang, Yun; Cunniff, Kerrianne; Nardone, Julie; Laiho, Asta; Tahiliani, Mamta; Sommer, Cesar A; Mostoslavsky, Gustavo; Lahesmaa, Riitta; Orkin, Stuart H; Rodig, Scott J; Daley, George Q; Rao, Anjana

Cell stem cell. 2011 Feb 04; 8(2):200-213

Ko, Myunggon; Huang, Yun; Jankowska, Anna M; Pape, Utz J; Tahiliani, Mamta; Bandukwala, Hozefa S; An, Jungeun; Lamperti, Edward D; Koh, Kian Peng; Ganetzky, Rebecca; Liu, X Shirley; Aravind, L; Agarwal, Suneet; Maciejewski, Jaroslaw P; Rao, Anjana

Nature. 2010 Dec 09; 468(7325):839-843

Huang, Yun; Pastor, William A; Shen, Yinghua; Tahiliani, Mamta; Liu, David R; Rao, Anjana

PLoS one. 2010 Jan 26; 5(1):e8888-e8888

Iyer, Lakshminarayan M; Tahiliani, Mamta; Rao, Anjana; Aravind, L

Cell cycle. 2009 Jun 01; 8(11):1698-1710