This laboratory studies the biosynthesis and sorting of two model proteins that are synthesized in membrane-bound ribosomes but have different subcellular destinations: 1) a rat liver microsomal esterase that is a permanent resident of the endoplasmic reticulum lumen and 2) a lysosomal membrane protein that is also in significant concentrations in the endosomal membranes. The goals of this research are to elucidate the nature of the signals and the sorting mechanisms that lead to the retention of some proteins in the endoplasmic reticulum and addresses other proteins to the lysosomal membrane. Using biochemical and immunocytochemical techniques, we work with cloning cDNAs for these proteins, constructing specific mutations, and analyzing the sorting of normal and mutated polypeptides expressed in transfected cells.
Associate Professor, Department of Cell Biology
Senior Associate Dean for Medical Education
Medical education online. 2017; 22(1):1396172-1396172
New England journal of medicine. 2013 Sep 19; 369(12):1085-1087
Academic medicine. 2010 Sep; 85(9 Suppl):S380-S386
Virtual mentor : VM. 2003 Dec 1; 5(12):?-?
Annals of neurology. 1998 Jul; 44(1):87-98
CHARACTERIZATION OF RAB BINDING-PROTEINS THAT MAY MEDIATE THE ACTIONS OF RAB11 AND RAB8 [Meeting Abstract]
Molecular biology of the cell. 1995 Nov; 6(23):690-690
Cell. 1994 Dec 30; 79(7):1157-1164