Bile acid synthesis is the major metabolic pathway for the elimination of cholesterol. In addition to the pathway for bile acids that begins in the liver and is augmented with interruption of the enterohepatic circulation, a regulatory pathway has recently been discovered that begins with the production of 27-hydroxycholesterol in the aortic endothelium, macrophages, and other tissues. Our National Institutes of Health-funded research program is directed at the delineation of all the intermediates in the regulatory pathway and the determination of their biologic effects. We have already shown that 27-hydroxycholesterol is a potent inhibitor of cholesterol synthesis and LDL receptor activity. We are now investigating other biologic effects that may also occur.
A critical enzymatic step in the regulatory pathway is 7a-hydroxycholesterol which is catalyzed by a novel P-450 enzyme recently identified in our laboratory. This enzyme not only modifies the biologic effects of 27-hydroxycholesterol but also prevents the formation of monohydroxy bile acids which are known cholestatic agents. Thus, a second aspect of our research program is the metabolic basis of cholestatic liver disease.
Research Professor, Department of Medicine
Research Professor, Department of Pediatrics
MD from Duke University
PhD from University of North Carolina
Bile Acid Composition Changes over 6 Months Following Fecal Microbiota Transplantation in Children with Recurrent C. difficile Infections [Meeting Abstract]
American journal of gastroenterology. 2016 Oct; 111:S453-S454
Steroids. 2015 Dec; 104:61-64
Advances in physiology education. 2014 Dec; 38(4):279-285
Journal of Alzheimer's disease. 2013; 33(3):881-888
Journal of Alzheimer's disease. 2013; 35(3):441-450
Journal of lipid research. 2012 Nov; 53(11):2469-2483
Steroids. 2012 May; 77(6):575-577
Oocyte maturation: Biologic role of sterol intermediates [Meeting Abstract]
Reproductive sciences (Thousand Oaks, Calif.). 2012 Mar; 19(3):393A-394A