The main focus of our research is proteins and their roles in cellular signaling events. Recent strides in molecular biology techniques have improved understanding of intracellular signal transduction. However, we feel that we can gain a more complete understanding of the dynamics of intracellular decision making by directly studying proteins or changes in small-molecule metabolites that occur in response to changes in proteins.
We mainly use mass spectrometry for this purpose because it provides a wide variety of information about protein structure from small amounts of protein. We conduct the majority of our work in collaboration with neuroscientists at NYU School of Medicine and other universities through our Mass Spectrometry Core for Neuroscience, which is funded by the National Institute of Neurological Disorders and Stroke (NINDS).
One of our goals is to identify proteins that interact with one another during specific signaling events. We have a particular interest in signal transduction in neurons, especially the neurotrophin-signaling pathways.
These experiments involve isolating protein complexes by immunoprecipitation or affinity chromatography, cleaving the protein with a specific protease such as trypsin, and then determining the resulting peptides’ amino acid sequences by quadrupole-Orbitrap tandem mass spectrometry. We then use the masses and tandem mass spectra—which contain sequence information—of the peptides to search genome databases and identify proteins.
To quantify the relative amounts of each protein participating in signaling complexes in stimulated and nonstimulated states, we use stable isotope coding methods, such as stable isotope labeling with amino acids in cell culture (SILAC) or tandem mass tags. With these methods we can identify and quantify thousands of proteins in a single experiment.
We also seek to identify post-translational modifications on proteins and their roles in cellular signaling. In the past, we focused on modifications of single proteins. More recently, we have identified and measured changes in tens of thousands of phosphorylation sites, or thousands of ubiquitination sites, in response to the addition of growth factors to cells or changes in disease status.
Professor, Department of Cell Biology
Director - Proteomics Core
PhD from Johns Hopkins University
Cell reports. 2017 Oct 31; 21(5):1267-1280
Nucleic acids research. 2017 Sep 19; 45(16):9398-9412
Nucleic acids research. 2017 Sep 19; 45(16):9810-9810
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Cell reports. 2017 Aug 15; 20(7):1717-1728
Using Nanoflow LC-MS/MS to Study Metabolic Changes in Low Grade Astrocytoma [Meeting Abstract]
Molecular & cellular proteomics. 2017 AUG; 16(8):S61-S61
PLoS biology. 2017 Jul 18; 15(7):e2002457-e2002457e2002457
Biochimica & biophysica acta. 2017 Jul 13; 1864(1):208-225