Research

The Dynlacht lab is focused on two major research efforts:

I. Novel approaches toward understanding the epigenetic and transcriptional controls governing mammalian differentiation

We use a combination of biochemical, genomic, and computational methods to dissect the epigenetic controls that govern cell cycle progression and mammalian differentiation. Using muscle differentiation as a model, we are attempting to unravel mechanisms that underlie the transition between growth and terminal differentiation. Using stem cells as muscle precursors, we are also attempting to reveal the epigenetic controls that determine whether a cell will adopt one mesodermal fate versus another.

Our laboratory has a twenty-plus year commitment to understanding the role of chromatin and gene expression in cell proliferation and differentiation using stem cell and breast cancer models. Toward this effort, we are seeking candidates experienced in molecular biology to develop novel approaches and technologies for analyzing protein-chromatin interactions and epigenetic control of differentiation. Expertise with chromatin, gene expression, or transcription preferred but not essential. The ideal candidate will help develop state-of-the-art and cutting-edge approaches toward understanding the role of protein interactions in assembly and activation of regulatory elements and gene activation and repression.

II. Understanding the biochemical mechanisms that underlie centrosome duplication and assembly of primary cilia.

We are also focusing on understanding the molecular controls that promote ciliogenesis and centrosome duplication, which play a pivotal role in cell cycle progression and mitosis. The primary cilium is a key signaling organelle that plays a key role in regulating cell growth and differentiation, and defects in this structure are associated with many human diseases. Yet the switching mechanisms that convert centrosomes that organize the mitotic spindle to a primary cilium from which it is derived are not known. We are interested in understanding the proteins that regulate centrosome dynamics, structure, and function, with particular emphasis on the proteins that promote the conversion of a centriole to primary cilium, a key crossroads for cell cycle exit and differentiation. We are interested in how defects in centrosomal and ciliary proteins promote human disease.

For more information, please look at specific projects under the “Research” tab of our website, and if you are interested, please contact us at brian.dynlacht@med.nyu.edu.