Boeke Lab Team
We are a collaborative team of expert faculty, postdoctoral fellows, graduate students, and research staff devoted to the study of synthetic genome engineering in yeast and mammalian systems.
Jef D. Boeke, PhD, DSc, founded and directs the Institute for Systems Genetics at NYU Langone Health. From 1985-2013, Dr. Boeke was on the faculty at Johns Hopkins University School of Medicine. Dr. Boeke received a BS in biochemistry summa cum laude in 1976 from Bowdoin College, and then earned a PhD in molecular biology from Rockefeller University in 1982, where he worked with Peter Model and Norton Zinder on the genetics of the filamentous phage. He did his postdoctoral work at The Whitehead Institute of MIT as a Helen Hay Whitney Postdoctoral Fellow with Gerald Fink.
Dr. Boeke discovered a major form of mobile DNA, based on reverse transcription of RNA. He coined the term “retrotransposition” to describe this process, common to virtually all eukaryotic genomes and now studied by a worldwide scientific community. His systems-level studies helped elucidate intricate molecular mechanisms involved in retrotransposition in yeasts, mice and humans.
In the area of Synthetic Biology, Dr. Boeke leads the international team synthesizing an engineered version of the yeast genome, Sc2.0, the first synthetic eukaryotic genome. In 2018, he launched the “Dark Matter Project” designed to better understand the “instruction manuals” that specify how human genes are expressed, using big DNA technology.
Hala Iqbal, PhD
My research interests lie at the interface of chemical biology, which I did my graduate degree in, and synthetic biology—specifically, in using synthetic biology tools to develop functional novel molecules. My current project involves the engineering of antibiotics in yeast. Using the SCRaMbLE system developed in the Boeke lab, I'm working to modify antibiotics with different combinations of tailoring enzymes to generate molecules that evade microbial drug resistance. My non-science interests including writing, feminism, and my newest obsession: rock climbing!
Luciana Stefanita-Lazar, PhD
My research topic focuses on understanding how species-specific molecular factors drive nuclear organization across organisms. In eukaryotes, the myriad of DNA-binding factors participates in the folding of the DNA molecule and its spatial reposition inside the nucleus. The resulting non-random 3D organization has proven to function in biological processes, however, the molecular mechanisms at its basis are still unclear. For example, higher-order chromatin structures can vary dramatically between eukaryotes suggesting that DNA and its binding factors have adapted to fulfill organism-specific functions. To understand the fundamental principles of small-to-large chromosome organization, I use Hi-C to characterize the 3D structure of artificially engineered yeast strains where the endogenous chromatin remodeling factors are replaced by the human counterparts.
Stephanie Lauer, PhD
I’m using synthetic biology to study fundamental questions about evolutionary processes. I’m interested in a specific subset of mutations: copy number variants and other large chromosomal rearrangements. I want to understand how these variants contribute to phenotypic variation within species, and, lead to reproductive isolation and speciation. I strongly believe budding yeast is a superior model organism, in part because of my enthusiasm for wine and craft beer. I also enjoy reading fiction, going to concerts, and yoga.
Weimin Zhang, PhD
I am trying to genetically engineer a stable haploid mouse embryonic stem cell line that could serve as a powerful platform for mammalian genome writing. On top of that, I am developing a genome writing strategy that enables big chunks of synthetic DNA integration in both haploid and diploid mouse embryonic stem cell lines. My ultimate goal is to generate a cancer-resistant stem cell line by minimizing the mutation rate of tumor suppressors. I am also a member of the Center of Excellence in Genomic Science team in the Boeke Lab, and I am interested in dissecting the risk variants in type 2 diabetes genomes. Outside of the lab, I like sports such as badminton and basketball.
Greg Goldberg, PhD
I am broadly interested in molecular and evolutionary mechanisms that shape the genomic content and phenotype of cellular populations over time. My research, co-mentored by Marcus Noyes, is aimed at rationally exploiting these mechanisms in the laboratory to facilitate the study of biological systems and the advancement of human health. Toward this end, I am currently developing novel CRISPR-based tools that provide users with additional control over the incorporation of genetic and epigenetic edits. Given that the phenotypic consequences of designer edits remain difficult to predict in certain contexts, I am also researching in vivo directed evolution methodologies that can expedite forward screening efforts to identify desirable edits in a genome or its functional components.
Wilson McKerrow, PhD
I am a computational biologist and probabilist, co-mentored by David Fenyo, who is interested in the causes and implications of retrotransposon expression in healthy and diseased tissues. Retrotransposons are DNA elements that copy their sequences to new genomic loci via an RNA intermediate. In addition to being a source of mutation and DNA damage, retrotransposon expression can lead to the induction of an innate immune response. I develop and apply algorithms to measure retrotransposon activity in “muti-omic” datasets and then identify the genomic and phenotypic correlates of that activity. Most recently, I have been using CPTAC genomic, transcriptomic and proteomic data to characterize LINE-1 retrotransposon expressing tumors.
Makiha Fukuda, PhD
I received my PhD in genome biology, which involved investigating the human “intergenic” region. My first postdoctoral fellowship focused on plant genomes specifically creating plant-animal fusion chromosomes. Here at the Boeke lab, my current project is to create RNA-based chromosomes in yeast as a way to investigate the origins, and perhaps even the future, of the genome. In my free time, I love to play the violin as well as go to concerts of all different genres.
Gudjon Olafsson, PhD
I am originally from Reykjavík, Iceland. I moved to London for my undergrad studies and later received my PhD from University College London and the Francis Crick Institute where I was mentored by Peter Thorpe, PhD. Prior to joining the Boeke Lab, I worked on phospho-regulation of chromosome segregation in budding yeast—in particular the kinetochore—at Queen Mary University of London. My research interests are mainly to utilize yeast as a tool to understand the fundamental mechanisms of chromosome segregation. The aim of my postdoctoral research in the Boeke Lab is to humanize the yeast kinetochore to illuminate the evolutionary divergence of eukaryotic kinetochores and to provide a novel solution to study the regulation of the complex human kinetochore in a relatively simple and tractable cellular model system. The ultimate goal is to use the humanized yeast to uncover mechanisms of human chromosome segregation which are important for cancer development.
Yu “Jeremy” Zhao
I am a graduate student in the Boeke lab working on synthetic biology. My current project is to build the first eukaryote with its whole genome redesigned and synthesized. I love hiking and biking. My favorite place in New York state is the Hudson Valley, especially in spring or autumn.
I was raised in Bangalore, India, then completed undergraduate degrees in molecular biology and philosophy at Johns Hopkins University. In general, my research interests are to build biological systems from the ground up in order to understand their function. My PhD work is co-supervised by Liam Holt. We are constructing embryonic stem cells bearing large pieces of synthetic DNA to investigate how genes are turned on and off through development. When I’m not wrangling DNA, I enjoy soccer, heavy metal guitar, food, and sharing the joys of a scientific worldview.
I’m fascinated by the genetic codes underlying human evolution, development and diseases. I did my undergraduate studies in biological sciences at China Agricultural University. Before joining the graduate program at NYU Grossman School of Medicine in 2015, I worked with Dr. Chengqi Yi at Peking University and developed chemical tools for genome-wide analysis of DNA epigenetic modifications. At NYU Langone, I’m fortunate to be co-mentored by Drs. Itai Yanai and Jef Boeke, allowing me to think broadly about our genome and its evolution. I’m combining both experimental approaches and computational tools to understand the genome innovation by point mutations and transposable elements. Ultimately, I hope to understand how integrated genetic changes impact human health and evolution.
Originally hailing from New Canaan, CT, I did my undergraduate work at MIT before joining NYU Grossman School of Medicine’s Medical Scientist Training Program. I became a member of the Boeke lab in 2017. My current research harnesses synthetic biology for both mammalian and yeast applications, running the gamut from cancer immunology and immunotherapy to synthetic yeast chromosome construction.
I am originally from Copenhagen, Denmark, but moved to Germany after high school to attend Jacobs University Bremen where I studied biochemistry and cell biology before coming to NYU Grossman School of Medicine for my PhD. I am passionate about the use of synthetic biology and metabolic engineering to solve societal problems whether medical, industrial or environmental. In my current work, I am engineering mammalian cells to be able to synthesize essential amino acids. Outside of the lab, you're likely to find me in the outdoor parks of New York City or at a rock-climbing gym.
I'm originally from Green Bay, Wisconsin, and attended college at the University of Wisconsin Madison. I joined the Boeke lab with interests in yeast molecular biology, gene regulation, and synthetic biology. I am currently tackling questions on human epigenetic machinery by reconstituting various components in the budding Saccharomyces cerevisiae. Outside of the lab, I enjoy reading, collecting vinyl records, playing video games, and brewing beer.
I’m from Ottawa, Canada, and previously attended the University of Ottawa. I joined the Boeke lab with an interest in synthetic biology and genome engineering. My major project in the lab uses synthetic genomics, taking advantage of our Big-DNA assembly technology, to study how genes are regulated by distal regulatory elements. My work is specifically focused on studying the alpha-globin locus and its associated “super-enhancer.” My interests outside of science include running, drumming, and hockey.
I grew up in New Jersey, and as a high school student I knew I wanted to go into biology research. I graduated from Rutgers University–New Brunswick with a degree in molecular biology and biochemistry and found my passion for research during my undergraduate years. I have always been interested in synthetic biology and how it can shape the future of science and medicine. My project is being co-supervised by Dr. Fenyö, and it involves genomic engineering methods developed by the Boeke Lab to provide insight on genetic risk variants involved in Type II diabetes. Outside of the lab, I enjoy drawing, painting, and finding new restaurants around New York City!
Senior Architect, Solution Development
Senior Research Scientist
Assistant Research Technician
Leslie Mitchell, PhD
Former Postdoctoral Fellow
David Truong, PhD
Former Research Fellow
Paolo Mita, PhD
Former Research Instructor
Jon Laurent, PhD
Former Postdoctoral Fellow