Giannarelli Lab investigators use single-cell technologies to map the cellular and molecular composition of human atherosclerotic plaques. We use a multi-tissue systems biology approach to infer arterial wall- and blood-specific immune networks that govern the immune response at different stages of human atherosclerosis. We integrate time-of-flight mass cytometry (CyTOF), single-cell RNA sequencing (scRNAseq), cellular indexing of transcriptomes and epitopes (CITE-Seq), and shotgun proteomics to measure immune cell variation in human samples. This data allows us to investigate the contribution of the immune system to cardiovascular disease and set the stage for the selection of molecular targets for novel anti-inflammatory therapies.