Cytometry & Cell Sorting Laboratory Protocols & Resources | NYU Langone Health

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Cytometry & Cell Sorting Laboratory Cytometry & Cell Sorting Laboratory Protocols & Resources

Cytometry & Cell Sorting Laboratory Protocols & Resources

NYU Langone’s Cytometry and Cell Sorting Laboratory follows strict laboratory protocols when analyzing and sorting cells.

Immunofluorescent Labeling

This excerpt from Current Protocols in Cytometry outlines some of the fundamental procedures for immunophenotyping using flow cytometry.

Sorting

We recommend several tips for a successful cell-sorting experience.

Live Cell Cycle

We offer this protocol for staining of live cells with Hoechst® 33342.

Fixed Cell Cycle and Apoptosis

We offer this protocol for staining of fixed cells using propidium iodide, Hoechst® 33342, or chromomycin A3.

Side Population

We recommend a Current Protocols in Cytometry article on side population isolation and analysis of stem cells.

Calcium Flux Assay

See this protocol for the ratiometric method using the Indo-1 stain for quantitation of intracellular calcium levels.

For discussion of a wider variety of cytometry protocols, please see the publication Current Protocols in Cytometry.

Common Products

Refer to this list of common products used in the lab.

Online Resources

In addition to common cell analysis and cell sorting protocols, you may find the following online resources helpful.

Experiment design tools:

Purdue University Cytometry Laboratories Discussion List

Metroflow, the New York/New Jersey Flow Cytometry Users Group

International Society for Advancement of Cytometry

Northeast Regional Life Sciences Core Directors

Association of Biomolecular Resource Facilities