Cytometry & Cell Sorting Laboratory Protocols & Resources
NYU Langone’s Cytometry and Cell Sorting Laboratory follows strict laboratory protocols when analyzing and sorting cells.
This excerpt from Current Protocols in Cytometry outlines some of the fundamental procedures for immunophenotyping using flow cytometry.
We recommend several tips for a successful cell-sorting experience.
Live Cell Cycle
We offer this protocol for staining of live cells with Hoechst® 33342.
Fixed Cell Cycle and Apoptosis
We offer this protocol for staining of fixed cells using propidium iodide, Hoechst® 33342, or chromomycin A3.
We recommend a Current Protocols in Cytometry article on side population isolation and analysis of stem cells.
Calcium Flux Assay
See this protocol for the ratiometric method using the Indo-1 stain for quantitation of intracellular calcium levels.
For discussion of a wider variety of cytometry protocols, please see the publication Current Protocols in Cytometry.
Refer to this list of common products used in the lab.
In addition to common cell analysis and cell sorting protocols, you may find the following online resources helpful.
Experiment design tools: