
Cytometry & Cell Sorting Laboratory Protocols & Resources
NYU Langone’s Cytometry and Cell Sorting Laboratory follows strict laboratory protocols when analyzing and sorting cells.
Immunofluorescent Labeling
This excerpt from Current Protocols in Cytometry outlines some of the fundamental procedures for immunophenotyping using flow cytometry.
Sorting
We recommend several tips for a successful cell-sorting experience.
Live Cell Cycle
We offer this protocol for staining of live cells with Hoechst 33342.
Fixed Cell Cycle and Apoptosis
We offer this protocol for staining of fixed cells using propidium iodide, Hoechst 33342, or chromomycin A3.
Side Population
We recommend a Current Protocols in Cytometry article on side population isolation and analysis of stem cells.
Calcium Flux Assay
See this protocol for the ratiometric method using the Indo-1 stain for quantitation of intracellular calcium levels.
For discussion of a wider variety of cytometry protocols, please see the publication Current Protocols in Cytometry.
Common Products
Refer to this list of common products used in the lab.
Online Resources
In addition to common cell analysis and cell sorting protocols, you may find the following online resources helpful.
Experiment design tools:
Purdue University Cytometry Laboratories Discussion List
Metroflow, the New York/New Jersey Flow Cytometry Users Group
International Society for Advancement of Cytometry