Rodent Genetic Engineering Laboratory
NYU Langone’s Rodent Genetic Engineering Laboratory is a resource for researchers who want to apply advanced molecular genetic techniques in rodent models of physiology and disease. Our lab is experienced at generating novel mutant mouse strains by using gene targeting in embryonic stem cells (ESCs) and by developing novel methods of modifying rodent genomes and assaying genetically modified cells in vivo.
To reserve time on an instrument, faculty, staff, and investigators must be registered in iLab. We have also provided iLab login instructions for NYU Langone, NYU, NYU College of Dentistry, and external users.
Our lab scientists customize technologies to meet individual project needs, including specialized expertise in the use the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) systems and other approaches for direct genome editing in vivo.
Available advanced technologies include CRISPR/Cas9, a proprietary rapid strain-creation method known as the EZ-Mouse Model, tetraploid complementation systems, GEMMs-ESC Speedy Models, regulated gene expression by high-throughput RNA interference assay in mice, safe-harbor knock-in gene editing using the Rosa26 and other loci, recombinase-mediated cassette exchange, Inducible Tet-On® and Tet-Off® Gene Expression Systems, homologous recombination, induced pluripotent stem cells, assisted reproductive technologies, and vitrification systems.
Additional traditional technologies we offer to staff, faculty, and external researchers are the production of transgenic mice by pronuclear injection of plasmid DNA or bacterial artificial chromosome DNA and the production of ESC chimeras by blastocyst injection. We also produce fully ESC-derived mice by tetraploid complementation, including following, isolating, and manipulating novel ESC lines from existing mouse strains.
The laboratory also offers sperm and embryo cryopreservation and storage technologies for strain preservation and recovery. Highly efficient in vitro fertilization technologies using fresh or cryopreserved sperm are available, facilitating the import of novel mouse strains without quarantine and recovery of poorly breeding strains. We also offer traditional embryo rederivation technologies for animal import into barrier vivaria through quarantine.
Our Rodent Genetic Engineering Laboratory locations are in the vivarium on the ground floor of Skirball Institute for Biomolecular Medicine; in room 334 of the Medical Science Building; and in the barrier vivarium on the second floor of the Alexandria Center for Life Science, West Tower.
How to Acknowledge the Rodent Genetic Engineering Laboratory
If you have used instruments, technologies, expertise, or data provided by NYU Langone’s Rodent Genetic Engineering Laboratory in research that is published in any form or used in grant applications, we appreciate being acknowledged. Please submit this acknowledgement form with your service request.
This shared resource is partially supported by the Cancer Center Support Grant P30CA016087 at the Laura and Isaac Perlmutter Cancer Center. Please acknowledge this grant in all publications resulting from the use of this facility. Additionally, all publications resulting from the use of this facility are required to abide by the National Institutes of Health Public Access Policy and be deposited in PubMed Central®