Hi-C Sequencing | NYU Langone Health

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Applied Bioinformatics Laboratories Services Hi-C Sequencing

Hi-C Sequencing

The Applied Bioinformatics Laboratories offer analysis of Hi-C sequencing, or Hi-C-seq, data. Chromatin conformation capture assays that are used to detect genome-wide DNA–DNA interactions.

Request a Hi-C-Seq Service

To request this service from us, please provide the following:

  • a sample sheet with the corresponding conditions for the samples
  • the organism(s) of the particular study
  • the restrictions enzyme(s) using for Hi-C library preparation
  • the link to the generated FASTQ data (obtained from NYU Langone’s Genome Technology Center or another sequencing facility)

You receive the following from us:

  • a comprehensive report
  • genome browser tracks for visualization with the WashU Epigenome Browser
  • a principal component analysis plot for assessment of sample variations
  • per-sample matrices with (normalized) interaction frequencies
  • per-sample matrices with topologically associating domain (TAD) boundary strength scores
  • per-sample lists of TAD calls
  • matrix of chromatin DNA–DNA interactions annotated by gene

Additional or Customized Analyses

We also provide the following analyses:

  • differential TAD activity analysis
  • TAD disruption analysis
  • integrative analyses with RNA-seq data
  • integrative analyses with H3K27ac, CTCF, or other ChIP-seq data
  • virtual 4C for selected loci

Each of these may be subject to an additional charge.

Additional Resources

Below is a link to a computational pipeline that we typically use for our analyses:

The following select publications have used our analyses: