Single-Cell RNA Sequencing | NYU Langone Health

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Applied Bioinformatics Laboratories Services Single-Cell RNA Sequencing

Single-Cell RNA Sequencing

The Applied Bioinformatics Laboratories perform single-cell RNA sequencing (RNA-seq) services. Single-cell RNA-seq is a genomic approach for the quantitative analysis of mRNA levels at a single cell resolution, allowing for transcriptome analysis of individual cells. This enables the evaluation of the level of heterogeneity within a single population or identification of rare subpopulations. It can also reveal co-expressed genes and uncover co-regulated gene modules.

Request a Single-Cell RNA-Seq Service

To request this service from us, please provide the following:

  • a sample sheet with the corresponding conditions for the samples
  • the organism(s) of the particular study
  • the link to the generated FASTQ data (obtained from NYU Langone’s Genome Technology Center or another sequencing facility)

You receive the following from us:

  • a comprehensive report
  • quality assessment (alignment rates, average number of genes, and transcripts)
  • visualization using t-SNE plots
  • clustering (unbiased classification of cells based on gene expression)
  • identification of cluster biomarkers
  • normalized expression values for all genes across all cells and clusters

Additional or Customized Analyses

We also provide the following analyses:

  • heatmaps for specific gene signatures
  • differential expression between different sample groups
  • cell differentiation/development trajectories

 Each of these may be subject to an additional charge.

Additional Resources

Below you can find links to the computational pipelines that we typically use for our analyses:

The following select publication has used our analyses: