The Applied Bioinformatics Laboratories offer 16S ribosomal RNA (rRNA) metagenomic sequencing services, a commonly used amplicon sequencing method to identify bacterial taxonomic composition within samples and compare microbial community differences between groups.
Request a Metagenomic Sequencing Service
To request this service from us, please provide the following:
- a sample sheet with the corresponding conditions for the samples
- the link to the generated FASTQ data (obtained from NYU Langone’s Genome Technology Center or another sequencing facility)
You receive the following from us:
- a complete taxonomic table
- alpha and beta diversity analysis
- a principal component analysis plot for assessment of sample variations
- a stack bar plot of bacterial relative abundance
- box plots of bacterial relative abundance for experimental groups
Additional or Customized Analyses
We also provide the following analyses:
- LEfSe analysis
- prediction of metagenome functional content
- supervised or unsupervised clustering of differentially bacterial taxa
- correlation network analysis
Each of these may be subject to an additional charge.
Below you can find links to the computational pipelines that we typically use for our analyses:
The following select publication has used our analyses:
- Tetz G, Ruggles K, Zhou H, Heguy A, Tsirigos A, Tetz V. Bacteriophages as potential new mammalian pathogens. Scientific Reports. 2017:7, 7043.