Experts at NYU Langone’s Genome Technology Center use the latest technologies in genome sequencing.

Illumina NovaSeq X-Plus

• highest throughput of any production platform
• lowest cost with more generated data

Available options:

• 1.5B flow cells with 100, 200, 300 cycles (2 lane flow cell)
• 10B flow cells with 100, 200, 300 cycles (8 lane flow cell)
• 25B flow cells with 100, 300 cycles (8 lane flow cell)

Single Cell library prep

• 10x platform for single cell RNAseq (3' and 5' protocol available)
• 10x multiome ATACseq & RNAseq from nuclei
• 10x visium platform for FFPE material
• 10x chromium connect for automated library prep
• Illumina single cell 3' prep (known as PIP seq) for more flexible and cheaper alternative

REVIO (PacBio)

• long read sequencing with methylation data (for PCR free protocol-SMRT 3.0)
• de novo genome sequencing for any kind of DNA (Plants and Animals)
• amplicon sequencing
• long reads single cell (10x genomics + Kinnex-PacBio)
• Kinnex for total RNA samples
• 16S long reads isoform

Oxford Nanopore Promethion P24

• long read sequencing with methylation data
• de novo genome sequencing
• amplicon sequencing
• 10x cDNA sequencing for long reads
• direct total RNA sequencing

Illumina MiSeq and MiSeq i100

• metagenomics
• 16S ribosomal RNA (rRNA) sequencing
• amplicon sequencing

Beckman Coulter Biomek SPRIworks HT

• automated library prep
• automated exome or targeted capture
• automated quantitative polymerase chain reaction (qPCR) setup

Automated library prep

• tecan fluent system
• Connect for 10× genomics library prep
• Opentrons flex system

Bio-Rad QX200 Droplet Digital PCR

• absolute quantification of RNA and DNA molecules
• rare or low-frequency variants or mutation
• pathogen detection

Covaris R230

• DNA and chromatin shearing for next-generation sequencing (NGS) library preparation in 96 well plate format

Agilent 2100 Bioanalyzer

• measures quantity and quality of RNA and DNA

Bio-Rad CFX384 Touch Real-Time PCR System

• library prep quantification (please note: we do not offer qPCR as a service)

Agilent 2200 & 4200 TapeStation

• automated benchtop system to measure the quality of biological samples from 2 to 96 samples at a time

Qubit 2.0 Fluorometer

• quantification of double-stranded DNA (dsDNA)