NYU Langone's Genome Technology Center (RRID: SCR_017929) provides faculty, staff, and partnering researchers with a range of services related to genome, epigenome, and transcriptome analysis. We offer numerous advanced technologies, including the following:

• Illumina sequencing (Nova X PLUS and MiSeq i100) and sample preparation for a variety of applications, including DNA and RNA sequencing (whole genome and RNA-seq), exome sequencing, targeted capture, chromatin confirmation, methylation (Methyl-seq), metagenomics, and many others
• Automation of library and targeted capture preps, including 16S ribosomal RNA (rRNA) sequencing
• Oxford Nanopore (P24) & PacBio sequencing (REVIO) for long reads sequencing.
• Bio-Rad Droplet Digital polymerase chain reaction (PCR)
• Single-cell RNA- and DNA-seq using 10x Genomics Chromium System and Illumina single cell 3’ prep (known as PIP seq)
• Combined strategy such as Long reads single cell (10x genomics + Pacbio)

Working closely with the Applied Bioinformatics Laboratories, we also provide expertise on the best strategies to help you achieve research goals in any field related to genomics, and we can tailor bioinformatics analysis to your individual project. If you supply us with nucleic acids, we can perform every step required to help you achieve your desired results.

What's New

Illumina NovaSeq X Plus is here. Please contact us to discuss your project.

Immune Reactions Found Behind Human Rejection of Transplanted Pig Kidneys

A new investigation by NYU Langone Health researchers explored the transplantation of a genetically engineered pig kidney into a brain-dead recipient, whose family donated his body to science. The investigators created a detailed map of both human and pig kidney immune activity in response to the transplant. They found that rejection was driven by antibodies—immune proteins that “tag” foreign substances for later destruction—as well as by T cells, which target and kill specific invaders. Researchers were able to reverse the rejection using a combination of drugs to temper both the antibody and T cell activity. The single-cell RNA sequencing and the spatial transcriptomics for this project were led by the Genome Technology Center; these technologies were key to the understanding of the immunological response in the pig-to-human kidney xenotransplantation setting. The study was published online in Nature.

Reserving Instruments Online

To reserve time on an instrument, faculty, staff, and investigators must be registered in iLab. We have also provided iLab login instructions for NYU Langone, NYU, NYU College of Dentistry, and external users.

New Sequencing Technologies

To best serve the research community, we’re continually evaluating novel technologies and acquiring new instruments. We offer a full range of single-cell RNA-seq solutions, including the 10x Genomics Chromium System and Illumina single cell 3’ prep (known as PIP seq). We have 3 Illumina NovaSeq X Plus and a MiSeq i100, a population-level high-throughput sequencer, and offer long-read technologies, including a PacBio Revio and the Oxford Nanopore PromethION (P24). We offer full support for all platforms.

Sequencing Results

To access sequencing results, you need approval from the relevant principal investigator and an account from the administrators of NYU's high-performance computing facility. Please ask the principal investigator to email hpc_admins@nyumc.org, indicating approval for your access and stating whether the access will be for one run or all of the runs from the lab.

Contact Us

NYU Langone Genome Technology Center
550 First Avenue
Medical Science Building 286
212-263-5743
genomics@nyulangone.org

How to Acknowledge the Genome Technology Center

Please use the RRID in all of your citations when using the core.

If you have used instruments, services, expertise, or data provided by NYU Langone’s Genome Technology Center (RRID: SCR_017929) in research that is published in any form or used in grant applications, we appreciate being acknowledged.

This shared resource is partially supported by the Cancer Center Support Grant P30CA016087 at the Laura and Isaac Perlmutter Cancer Center. Please acknowledge this grant in all publications resulting from the use of this facility. Additionally, all publications resulting from the use of this facility are required to abide by the National Institutes of Health Public Access Policy and be deposited in PubMed Central.

Support for use of our resources may be available upon application to NYU Langone’s Clinical and Translational Science Institute (CTSI). See more information about how to apply for support. If CTSI support is obtained, the following manuscript citation is suggested: “Supported in part by grant UL1 TR001445 from the National Center for Advancing Translational Sciences, National Institutes of Health.”

How to Acknowledge the National Institutes of Health S10 Support

If your project involves PacBio Sequel data, please acknowledge the National Institutes of Health Shared Instrumentation Grant 1S10OD023423-01.