Genome Technology Center
NYU Langone's Genome Technology Center provides faculty, staff, and partnering researchers with a range of services related to genome, epigenome, and transcriptome analysis. We offer numerous advanced technologies, including the following:
- Illumina deep sequencing and sample preparation for a variety of applications, including DNA and RNA sequencing (DNA- and RNA-seq), exome sequencing, targeted capture, chromatin immunoprecipitation sequencing (ChIP-seq), methylation sequencing (Methyl-seq), metagenomics, and many others
- Automation of Illumina library and targeted capture preps, including 16S ribosomal RNA (rRNA) sequencing
- Oxford Nanopore sequencing (long reads)
- Bio-Rad Droplet Digital™ polymerase chain reaction (PCR)
- Nanostring nCounter
- Single-cell RNA- and DNA-seq using the C1™ Auto Prep System from Fluidigm, and 10x Genomics Chromium™ System
Working closely with the Applied Bioinformatics Laboratories, we also provide expertise on the best strategies to help you achieve research goals in any field related to genomics, and we can tailor bioinformatics analysis to your individual project. If you supply us with nucleic acids, we can perform every step required to help you achieve your desired results.
Illumina NovaSeq 6000 is here. Please contact us to discuss your project.
Karyotype Engineering by Chromosome Fusion Leads to Reproductive Isolation in Yeast
Jef Boeke, PhD, and his study team were able to generate yeast strains that have far fewer chromosomes—some with as little as two—even if they have the same amount of genetic information. The researchers found that fusing together chromosomes only had a modest effect on overall transcriptome. The engineered yeast species grew without major defects, although they were now reproductively isolated—findings that speak to the robustness of the genome and provide clues to origins of speciation. The creation of deep sequencing libraries for this important work was supported by Adriana Heguy, PhD, and Paul Zappile, MS, from the Genome Technology Center. The study was published online in Nature.
Reserving Instruments Online
To reserve time on an instrument, faculty, staff, and investigators must be registered in iLab. We have also provided iLab login instructions for NYU Langone, NYU, NYU College of Dentistry, and external users.
New Sequencing Technologies
To best serve the research community, we’re continually evaluating novel technologies and acquiring new instruments. We offer a full range of single-cell RNA-seq solutions, including the 10x Genomics Chromium™ System and the 1CellBio InDrop™ System. We have an Illumina NovaSeq 6000, a population-level high-throughput sequencer, and offer long-read technologies, including a PacBio Sequel and the Oxford Nanopore PromethION (beta testing). We offer full support for all platforms.
To access sequencing results, you need approval from the relevant principal investigator and an account from the administrators of NYU's high-performance computing facility. Please ask the principal investigator to email email@example.com, indicating approval for your access and stating whether the access will be for one run or all of the runs from the lab.
The Genome Technology Center is located at 550 First Avenue, in Medical Science Building 303–308. To inquire about our capabilities and services, please email us at firstname.lastname@example.org or call us at 212-263-8048. To reach Adriana Heguy, PhD, director, email email@example.com.
How to Acknowledge the Genome Technology Center
If you have used instruments, services, expertise, or data provided by NYU Langone’s Genome Technology Center in research that is published in any form or used in grant applications, we appreciate being acknowledged.
This shared resource is partially supported by the Cancer Center Support Grant P30CA016087 at the Laura and Isaac Perlmutter Cancer Center. Please acknowledge this grant in all publications resulting from the use of this facility. Additionally, all publications resulting from the use of this facility are required to abide by the National Institutes of Health Public Access Policy and be deposited in PubMed Central®.
Support for use of our resources may be available upon application to NYU Langone’s Clinical and Translational Science Institute (CTSI). See more information about how to apply for support. If CTSI support is obtained, the following manuscript citation is suggested: “Supported in part by grant UL1 TR001445 from the National Center for Advancing Translational Sciences, National Institutes of Health.”
How to Acknowledge the National Institutes of Health S10 Support
If your project involves PacBio Sequel data, please acknowledge the National Institutes of Health Shared Instrumentation Grant 1S10OD023423-01.