Electron Microscopy Technologies & Instruments | NYU Langone Health

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Microscopy Laboratory Electron Microscopy Technologies & Instruments

Electron Microscopy Technologies & Instruments

NYU Langone’s Microscopy Laboratory uses powerful electron microscopes to obtain high-resolution images of your specimens. Electron microscopy sample preparation is generally a drop-off service. However, if you prefer to perform your own imaging and are a new user, we can provide training. If you are an experienced user, we can offer—with approval—the materials required for your experiment. See our fees for technologies and instruments.

All required forms are available in iLab.

Electron Microscopy Technologies

Our in-house electron microscopy technologies include the following:

  • embedding using either epoxy or acrylate-based embedding resins at ambient or low temperatures
  • thin sectioning using Leica Ultracut UCT and EM UC6/EM6 cryo-ultramicrotome
  • cryo-ultramicrotomy using Leica UC6/EM6 and UC7/EM7 cryo-ultramicrotome
  • array tomography sectioning using a Leica ARTOS 3D microtome
  • immunolabeling following wholemount, pre- or postembedding protocols
  • Tokuyasu immunogold labeling of ultrathin cryosections
  • high-pressure freezing using a Bal-Tec HPM 010, and freeze substitution by a Leica EM AFS2 automatic freeze-substitution system
  • negative staining of viruses, bacteria, and macromolecules
  • rotary shadowing using a BOC Edwards Auto 306 evaporator for DNA, RNA, and macromolecules
  • cryo EM or electron tomography data collection using either ThermoFisher Talos120C or JEOL1400 Flash transmission electron microscope (TEM)
  • three-dimensional electron microscopy using a Zeiss Gemini300 field-emission scanning electron microscope (SEM) with Gatan 3View® or a ThermoFisher Helios 650 DualBeam SEM (located at the New York Structural Biology Center)
  • correlative light and electron microscopy

Electron Microscopy Instruments

We provide access to some of the most powerful electron microscopy instruments:

  • FEI Talos L120C TEM with Gatan 4k x 4k OneView camera
  • JEOL1400 Flash TEM with Gatan 4k × 4k Rio CMOS camera and Gatan Elsa cryoholder
  • Gatan Model 655 turbo pumping station
  • Fischione 2040 dual-axis rotating holder
  • Zeiss Gemini 300 FESEM with Gatan 3View®
  • Leica Ultracut UCT microtome
  • Leica EM UC6/EM FC6 cryo-ultramicrotome
  • Leica ARTOS 3D microtome/FC7 cryo-ultramicrotome
  • Leica EM FCS cryo-ultramicrotome
  • Leica glass knife maker
  • BOC Edwards Auto 306 evaporator
  • PELCO BioWave® Pro laboratory microwave system
  • PELCO easiGlow
  • Rave Scientific CCU-010 HV coating system
  • Tousimis Autosamdri®-931 critical point dryer
  • Denton Desk V sputter coater
  • Zeiss Axio upright microscope with AxioCam ERc 5s digital camera