Light Microscopy Technologies & Instruments
For investigators needing assistance with light microscopy, NYU Langone’s Microscopy Laboratory offers competitive rates on experimental design, consultation, imaging assistance, instrument training, and access to image-acquisition equipment and data-analysis software packages.
All required forms are available in iLab.
Light Microscopy Instruments
The Microscopy Laboratory has a variety of microscopes to address many imaging needs.
Zeiss Axioplan Microscope (Upright)
The Zeiss Axioplan upright microscope is a bright-field microscope with additional differential interference contrast and polarized light functions. It is ideal for many types of specimen preparation, such as hematoxylin and eosin staining, periodic acid–Schiff staining, trichrome staining, and diaminobenzidine staining.
Zeiss Axio Observer Epifluorescence and Crossed-Polarization Microscope (Inverted)
The Zeiss Axio Observer epifluorescence and crossed-polarization microscope (inverted) is a high-end epifluorescence imaging system with multiple channels, Z stacks with Apotome, time series, and automated stage for multiposition or mosaic scans. It offers full environmental control and autofocus for long time-lapse imaging. It features an additional color camera for bright-field color-stained samples and for crossed polarization. We have an up-to-date license for the Zeiss ZEN (blue) software.
Applied Precision PersonalDV Live-Cell Imaging System (Inverted)
The Applied Precision PersonalDV live-cell imaging system (inverted) is a multidimensional imaging microscope for fixed and live samples. We offer an on-site deconvolution function to generate confocal-like images. It has Förster resonance energy transfer (FRET)-sensitized emission analysis for CFP/YFP pairs.
Nikon Eclipse Ti Total Internal Reflection Fluorescence/Epifluorescence Microscope (Inverted)
The Nikon Eclipse Ti total internal reflection fluorescence/epifluorescence microscope (inverted) is two scopes in one with full environmental chamber and automated stage.
First, it offers total internal reflectance fluorescence microscopy with an electron multiplying charged-coupled device camera (EMCCD) or scientific complementary metal-oxide semiconductor (sCMOS) camera. This allows a defined volume image that is less than 200 nm deep at the substrate.
Second, it offers wide-field fluorescence microscopy using discrete color LEDs for excitation and independently addressable filters for emission. This is advantageous for live work, high speed, or long time lapse. Ratio FRET can be done on both live and fixed material. We have an up-to-date license for the Nikon NIS-Elements software.
Zeiss LSM 700 Laser Scanning Confocal Microscope (Inverted)
The Zeiss LSM 700 laser scanning confocal microscope (inverted) offers laser scanning confocal imaging with environmental chamber. The scope is located in the Alexandria Center for Life Science East Tower and is used routinely for fixed- and live-sample imaging.
Zeiss LSM 710 Laser Scanning Confocal and Multiphoton Microscope (Inverted)
The Zeiss LSM 710 laser scanning confocal and multiphoton microscope (inverted) offers two imaging systems built on a Zeiss Axio Observer temperature-controllable microscope. An optional objective inverter allows it to function as an upright system.
The LSM 710 uses a Spectra-Physics Mai Tai® laser DeepSee™ gross dispersion compensator and five detectors for multidimensional intravital imaging on animal models or for quick screening of fixed tissue. It offers a standard or spectral confocal with a wide range of laser choices. It can also be used for photoactivation, fluorescence recovery after photobleaching (FRAP), and FRET.
Zeiss LSM 880 Laser Scanning Confocal Microscope with FLIM and FCS (Inverted)
The Zeiss LSM 880 laser scanning confocal microscope with fluorescence lifetime imaging (FLIM) and fluorescent correlation spectroscopy (FCS) is the newest and most comprehensive confocal system in the lab. It offers a standard confocal with up to 36 channels using two photomultiplier tubes and a 34-channel spectral detector. It has a wide range of lasers, from 405 nm through 638 nm.
It has two additional gallium arsenide phosphide (GaAsP) detectors for low-light imaging, FCS, and time-resolved FLIM using a 445-nm pulsed laser. It has an Airyscan detector to boost spatial resolution up to 1.7 times finer than standard confocal imaging. It can also be used for photoactivation, FRAP, and FRET. We have up-to-date licenses for Zeiss ZEN (blue) and PicoQuant SymPhoTime software.
Leica TCS SP5 II Confocal Microscope (Upright)
The Leica TCS SP5 II confocal microscope (upright) is shared with the developmental genetics group at Skirball Institute of Biomolecular Medicine. It is perfect for samples fixed on slides or live samples that can be imaged by dipping the lens in media. It offers a wide range of lasers, and two additional Leica HyD hybrid detectors make this system work multidimensionally under most conditions. Advanced light microscopy technologies such as FRAP, FRET, spectral imaging, and photon counting are also available with this system.
Olympus SZX10 Stereomicroscope
The Olympus SZX10 stereomicroscope is a standard dissection microscope for bright-field or epifluorescent samples.
Zeiss Lightsheet Z.1 Microscope
With the support of Howard Hughes Medical Institute and NYU Langone, the Zeiss Lightsheet Z.1 microscope system was installed in early 2016. It is excellent for transparent live organisms such as embryos and zebrafish and for small cleared organs, and complements confocal microscopy by imaging optical sections of larger tissues.
The light-sheet microscope works with living samples in water or media and with fixed and cleared samples, such as those treated with CLARITY. With lasers at 405, 488, 568, and 638 nm, the system images most genetically encoded fluorescent proteins and other popular fluorescent probes. Magnification is at 5× NA 0.16 or 20× NA 1.0. Images are collected with two 1920 × 1920–pixel sCMOS cameras.
Dell Precision Workstation and Image J
The Dell Precision workstation and National Institutes of Health (NIH) Image J offer high-performance image data analysis, especially with large data sets. Image J is a Java-based freeware from NIH and is widely used in microscopy for image processing and analysis. We encourage all users to get familiar with this software, and we offer training, assistance, and programming to investigators.
PerkinElmer Volocity Software
For users who need three-dimensional reconstruction, processing and quantification, or deconvolution of image data, we provide floating licenses of PerkinElmer Volocity software for use on most computers within the NYU Langone firewall.
Bitplane Imaris Software
In our lab, the old license is running on Windows XP for cell tracking and three-dimensional image analysis.