Light Microscopy Technologies & Instruments | NYU Langone Health

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Microscopy Laboratory Light Microscopy Technologies & Instruments

Light Microscopy Technologies & Instruments

For investigators needing assistance with light microscopy, NYU Langone’s Microscopy Laboratory offers competitive rates on experimental design, consultation, imaging assistance, instrument training, and access to image-acquisition equipment and data-analysis software packages.

All required forms are available in iLab.

Confocal and Two-Photon Microscopy

Our lab has a variety of confocal and two-photon microscopes to address many imaging needs.

Zeiss LSM 700 Laser Scanning Confocal Microscope (Inverted)

The Zeiss LSM 700 laser scanning confocal microscope (inverted) offers laser scanning confocal imaging with environmental chamber. The scope is located in the Alexandria Center for Life Science East Tower and is used routinely for fixed- and live-sample imaging.

Zeiss LSM 710 NLO Laser Scanning Confocal and Multiphoton Microscope (Inverted)

The Zeiss LSM 710 laser scanning confocal and multiphoton microscope (inverted) offers two imaging systems built on a Zeiss Axio Observer temperature-controllable microscope. An optional objective inverter allows it to function as an upright system.

The LSM 710 uses a Spectra-Physics Mai Tai laser DeepSee gross dispersion compensator and five detectors for multidimensional intravital imaging on animal models or for quick screening of fixed tissue. It offers a standard or spectral confocal with a wide range of laser choices. It can also be used for photoactivation, fluorescence recovery after photobleaching (FRAP), and FRET. The scope is located in the Alexandria Center for Life Science West Tower.

Zeiss LSM 880 Laser Scanning Confocal Microscope with FLIM and FCS (Inverted)

The Zeiss LSM 880 laser scanning confocal microscope with fluorescence lifetime imaging (FLIM) and fluorescent correlation spectroscopy (FCS) is the newest and most comprehensive confocal system in the lab. It offers a standard confocal with up to 36 channels using a photomultiplier tubes, a 34-channel spectral detector, and a red-shifted GaAsP detector. It has a wide range of lasers, from 405 nm through 638 nm.

It has two additional gallium arsenide phosphide (GaAsP) detectors for low-light imaging, FCS, and time-resolved FLIM using a 445-nm pulsed laser. It has an Airyscan detector to boost spatial resolution up to 2 times finer than standard confocal imaging. It can also be used for photoactivation, FRAP, and FRET. We have up-to-date licenses for Zeiss ZEN (blue) and PicoQuant SymPhoTime software. This scope is located at Skirball 2nd floor.

Leica TCS SP8 Confocal Microscope (Upright)

The Leica TCS SP8 confocal microscope (upright) is perfect for samples fixed on slides or live samples that can be imaged by dipping the lens in media. It offers a wide range of lasers, and additional Leica HyD hybrid detectors make this system work multidimensionally under most conditions. Advanced light microscopy technologies such as FRAP, FRET, spectral imaging, and photon counting are also available with this system. This scope is located at Skirball 4th floor.

Olympus Fluoview Multiphoton Microscope

The Olympus Fluoview multiphoton microscope has two lasers for excitation from 690 to 1300 nm, both galvos and a resonant scanner, and four detectors for multichannel deep fluorescence microscopy in live tissue. The microscope is located at Science Building 4th floor.

Widefield Microscopy

Our lab is equipped with a variety of widefield microscopes.

Zeiss Axio Observer Epifluorescence and Crossed-Polarization Microscope (Inverted)

The Zeiss Axio Observer epifluorescence and crossed-polarization microscope (inverted) is a high-end epifluorescence imaging system with multiple channels, Z stacks with Apotome, time series, and automated stage for multi-position or mosaic scans. It offers full environmental control and autofocus for long time-lapse imaging. It features an additional color camera for bright-field color-stained samples and for crossed polarization. We have an up-to-date license for the Zeiss ZEN (blue) software. This scope is located at Skirball 2nd floor.

Nikon Eclipse Ti Epifluorescence Microscope (Inverted)

The Nikon Eclipse Ti epifluorescence microscope (inverted) offers wide-field fluorescence microscopy using discrete color LEDs for excitation and independently addressable filters for emission. This is advantageous for live work, high speed, or long time lapse. Ratio FRET can be done on both live and fixed material. This scope is located at Skirball 3rd floor.

Toggel FLIM Camera on Nikon Eclipse TE2000 Microscope (Inverted)

The Toggel FLIM camera on Nikon Eclipse TE2000 microscope (inverted), with excitation of fluorescent molecules at 445, 488, 515, and 561 nm, directly images widefield microscopy as fluorescence lifetime on a pixel-by-pixel basis. The camera on an inverted fluorescent microscope simplifies FRET assays. In live-cell experiments, the built-in time-lapse function tracks changes of fluorescence lifetime over time. This scope is located at Smilow 1208A.

Zeiss Axioplan Microscope (Upright)

The Zeiss Axioplan upright microscope is a bright-field microscope with additional differential interference contrast and polarized light functions. It is ideal for many types of specimen preparation, such as hematoxylin and eosin staining, periodic acid–Schiff staining, trichrome staining, and diaminobenzidine staining. This scope is located at Skirball 3rd floor.

Zeiss Axio Zoom.V16 Fluorescent Stereomicroscope

Fully automatic controlled zoom scope, it offers bright fluorescence as well as transmitted light in large fields, optimized zoom for many applications. It is equipped with 1x and 2.3x Plan-Neofluar objectives, four fluorescent filter blocks, Zeiss AxioCam and motorized stage, fit most imaging requirements of large sections and whole mount tissues.

Zeiss Elyra 7 with Lattice SIM

Installed in 2020, the inverted Zeiss Elyra 7 microscope has three high resolution imaging modes. Single molecule localization microscopy (SMLM) modules for Stochastic Optical Reconstruction Microscopy (STORM) and Photoactivated Localization Microscopy (PALM) with lasers at 488, 561 and 640 nm delivers molecular resolution in large 3D volumes and powerful post-processing algorithms for quantification. Structured illumination with Lattice pattern offers 2x special resolution of confocal or conventional fluorescent microscopes. Standard Total Internal Reflectance Fluorescence microscopy (TIRF) allows imaging of fluorescent molecules within 200nm of substrate. Equipped with full environmental control for time lapse of live samples. This scope is located at Skirball 2nd floor.

Zeiss Lightsheet Z.1 Microscope

Zeiss Lightsheet Z.1 microscope system is excellent for transparent live organisms such as embryos and zebrafish, and for small cleared organs. It complements confocal/multiphoton microscopy by imaging larger tissues. The lightsheet microscope works with living samples in water or media and with fixed and cleared samples both aqueous and solvent-based media. With lasers at 405, 488, 568, and 638 nm, the system images most genetically encoded fluorescent proteins and other popular fluorescent probes. Magnification is at 2.5x NA 0.12, 5× NA 0.16 or 20× NA 1.0. Images are collected with two 1920 × 1920–pixel sCMOS cameras. This system was upgraded in 2019 with a Mesoscale Imaging System for larger and organic solvent-based imaging medium, and further upgraded to new workstation and graphics card in fall of 2021. This scope is located at Skirball EM Suite.

Thermo Scientific CellInsight CX7 LZR High Content Analysis Platform

This system installed in 2019 comes paired with an Orbitor RS automatic plate loader and on-stage incubator for high throughput and high content imaging. Widefield and spinning disk confocal imaging modes are available to perform robust screening of multiwell plates and glass slides. It is an ideal imaging platform for 3D organoid and spheroid imaging. The system features four excitation wavelengths at 405nm, 488nm, 561nm and 638nm to cover a wide gamut of fluorescent probes. Six objectives are available to meet rapid plate scanning collections and high resolution imaging for later analysis: 2xNA0.08; 4x0.18NA; 10xNA0.40; 20xNA0.45; 20xNA0.70; 40xNA0.60. Built-in image analysis software allows for online quantification and statistics during imaging. Stage features built-in incubation with temperature and CO2 control to allow for long term kinetics assays of live samples. This scope is located at Skirball 3rd floor.

Dell Precision Workstations and Image Analysis

We offer three high-end Dell Precision workstations that support quantitative large-volume data processing with Bitplane Imaris, ThermoFisher Amira, ORS Dragonfly, NIH ImageJ, IMOD, and other software.

We also offer full service experimental design, training, analysis, and presentation for publication.