Bioluminescence & Fluorescence Optical Imaging Scanners | NYU Langone Health

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Preclinical Imaging Laboratory Instruments Bioluminescence & Fluorescence Optical Imaging Scanners

Bioluminescence & Fluorescence Optical Imaging Scanners

We have three optical imaging scanners.

PerkinElmer IVIS Lumina III XR

A PerkinElmer IVIS® Lumina III XR is available in the Medical Science Building in the 481B area. The scanner is capable of imaging fluorescent and bioluminescent reporters or dye. Multiple subjects can be imaged within a light-tight and shielded imaging chamber on a heated sample stage.

The system comes with complete animal setup and handling to achieve noninvasive in vivo imaging within minutes under accurate physiological conditions (temperature-controlled heated stage, gas anesthesia, and syringe injection system for simultaneous compound administration).

The PerkinElmer IVIS® Lumina III XR is designed to enable flawless combination of optical and X-ray imaging. The system is equipped with Living Image® software that facilitates a precise overlay to visualize bioluminescence or fluorescence map activities with anatomical surface or X-ray features.

The X-ray setup is composed of a tungsten-anode X-ray source (40 kVp, 100 mA), an automated aluminum filter, and a thallium-activated cesium iodide scintillator. The fully computer-controlled system is equipped with a cooled CCD camera (–90 degree Celsius) and a 150-W tungsten/halogen intensity lamp, as well as a set of interchangeable emission filters customized for in vivo imaging with high intensity from 300 to 900 nm.

PerkinElmer IVIS Lumina III

A PerkinElmer IVIS® Lumina III is available in the Mispro vivarium within the specific-pathogen-free (SPF) barrier, located in the Alexandria Center for Life Science. This instrument allows us to serve many different biomedical researchers, including the tumor biology group.

The scanner is capable of imaging both fluorescent and bioluminescent reporters or dye. Multiple subjects can be imaged within a light-tight and shielded imaging chamber on a heated sample stage. The system is equipped with complete animal setup and handling to achieve noninvasive in vivo imaging within minutes under accurate physiological conditions (temperature-controlled heated stage, gas anesthesia, and syringe injection system for simultaneous compound administration).

The fully computer-controlled system is equipped with a cooled CCD camera (–90 degree Celsius) and a 150-W tungsten/halogen intensity lamp, as well as a set of interchangeable emission filters customized for in vivo imaging with high intensity from 300 to 900 nm.

PerkinElmer IVIS Spectrum

A PerkinElmer IVIS® Spectrum is housed in the procedure room of the vivarium at Skirball Institute of Biomolecular Medicine. The PerkinElmer IVIS® Spectrum is capable of three-dimensional imaging using either fluorescent or bioluminescent reporters. Single-subject three-dimensional tomographic scanning or simultaneous multiple-mouse two-dimensional acquisitions can be performed through a set of high-efficiency filters and spectral unmixing algorithms across the visible light spectrum to the near-infrared wavelength.

The instrument is equipped with 10 30-nm bandwidth excitation filters and 18 20-nm bandwidth emission filters that significantly reduce autofluorescence through the spectral scanning of filters and the use of spectral unmixing algorithms. The spectral unmixing tool enables the separation of signals from multiple fluorescent reporters within the same animal.

The PerkinElmer IVIS® Spectrum allows researchers to perform excitation from either the bottom (transillumination), for deep-tissue examination, or the top (epi-illumination) to illuminate in vivo fluorescent probes. The three-dimensional tomographic acquisition is helpful in determining the source localization and concentration using the combination of structured light and transillumination fluorescent image datasets.

The system is equipped with complete animal setup and handling to achieve noninvasive in vivo imaging within minutes under accurate physiological conditions (temperature-controlled heated stage, gas anesthesia, and syringe injection system for simultaneous compound administration).