Experimental Pathology Research Laboratory Technologies & Fees
At NYU School of Medicine’s Experimental Pathology Research Laboratory, our experts use advanced technologies to perform in-house services such as tissue preparation, embedding, sectioning, histochemical staining and immunohistochemistry, antibody optimization and validation, tissue microarray construction, and digital whole-slide scanning.
Additionally, many of our state-of-the-art instruments are available for rental through our laboratory information management system (LIMS), including instruments for embedding, sectioning, imaging, and laser capture microscopy.
We provide consultation and guidance to help you plan complex experiments, as well as informal training for standard procedures and instrument rental. If this is your first time working with us, please contact Cynthia Loomis, MD, PhD, director, at firstname.lastname@example.org, or Luis Chiriboga, PhD, technical director, at email@example.com, before starting a project.
All histology technologies are performed on-site with an average turnaround time of 10 to 15 working days.
The Experimental Pathology Research Laboratory has two automated high-throughput processors for paraffin processing of human and animal tissues. We also offer cryopreservation and preprocessing technologies, including perfusion and immersion fixation, decalcification, basic tissue grossing, and cassetting.
Paraffin embedding, cryoembedding, and agarose embedding technologies are available for human and animal tissues. Of note, we use separate paraffin embedders for human and animal tissues. Cryoembedding is performed using a liquid nitrogen–chilled isopentane bath to ensure minimal ice crystal formation. Cryoembedding tools, as well as our separate human and animal paraffin embedders, are available for rental.
Our experts offer paraffin sectioning, frozen sectioning (fixed or fresh), and vibratome sectioning. Sections can be stained or prepared for downstream DNA, RNA, or protein extraction and analysis, if desired. We also offer nondecalcified bone sectioning on a cryostat using a modified Kawamoto cryofilm method. Our microtomes and cryostats are available for rental.
Tissue Microarray Construction
In addition to traditional embedding technologies, we offer tissue microarray construction using formalin-fixed, paraffin-embedded samples. We can prepare your tissue microarray for use in collecting immunohistochemical and other histopathological data.
Staining is performed on-site with an average turnaround time of 10 to 15 working days.
In addition to standard hematoxylin and eosin staining, we offer a variety of special histological stains, including trichrome, oil red O, picrosirius red, and others.
Immunohistochemistry and Antibody Optimization and Validation
Our experts offer extensive options for routine and special immunohistochemical labeling, including multicolor chromogenic and immunofluorescence detection. We use manual labeling as well as automated high-throughput instruments. Our lab uses a standardized approach to optimize antibodies. Tissue samples are tested under a variety of conditions and treatments with the goal of producing high signal-to-noise performance for the assay.
We maintain a database of all antibodies tested in our lab. The database currently has more than 1,500 antibodies that have been tested against a wide variety of targets in both human and animal tissues.
RNA In Situ Hybridization
We use highly sensitive and robust protocols for performing chromogenic and fluorescence RNA in situ hybridization assays (using RNAscope® technology). This approach is an alternative strategy for determining biomarker oppression status and validating large genomic screens.
You can reserve our powerful imaging platforms between 9:00AM and 5:30PM, Monday through Friday. If you have already completed training on a particular instrument, you may be able to access our lab after hours and on weekends.
We provide several basic microscope systems for you to use, including two brightfield upright scopes, one with Nomarski (polarizing) optics; a fluorescence upright scope; and a fluorescence stereomicroscope. All microscopes are outfitted with digital cameras.
We provide high-resolution digital whole-slide scanning for both brightfield and fluorescence systems using up to four colors. You can view, save, and annotate images using a web-accessible digital image database.
Laser Capture Microdissection
Our expert staff provides training on the state-of-the-art Leica microdissection system, which can select specific cell types from tissue sections for downstream molecular analyses. The system, which has brightfield and fluorescence capabilities, is available to researchers for rental on a self-serve basis after they’ve completed required training.
Upon special request, we also offer full-service laser capture microdissection.
We were awarded a National Institutes of Health shared instrumentation grant for the PerkinElmer Vectra®, a quantitative high-throughput imaging system that overcomes many of the technical difficulties that can arise with the use of many other tissue-imaging systems.
The Vectra® accurately and sensitively resolves both multiplexed fluorescence probes and brightfield absorbance dyes in intact tissues, even when the signals overlap spectrally. The system can subtract background tissue autofluorescence, which is critical when assessing low-abundance protein targets or performing RNA fluorescence in situ hybridization. Although the Vectra® is not yet available for rental, we offer full-service multispectral imaging.
We also provide basic image analysis and training on our inForm® software platform.
The components of pricing for each technology includes the cost of consumables, the cost for technician time, and a small percentage of any associated service contracts. As with many of our technologies, the incremental cost of each procedure is affected by overall volume. As volume increases, we pass the resulting savings on to users in the form of reduced prices.
Please note that rush surcharge is double the price of all technologies.