Ion Channels & Immunity Program Core Facility & Resources

The Ion Channels and Immunity core facility will be part of the new Ion Channel and Immunity Program at NYU Langone and is meant to facilitate research in the area of ion channel function in immune cells.

The core facility will be available to all investigators in the NYU Langone community, regardless whether they have prior experience in measuring ion channel currents and fluxes. The core facility will also be available to collaborating investigators within the larger NYU community.

The day-to-day operations of the core facility will be led by a full-time PhD-level research scientist and additional research staff who can assist with experimental design, implementation, and training.

The core facility will be located on the NYU Langone Health main campus and will be available for service Monday through Friday from 9:00AM to 6:00PM.

To help plan which services the facility should provide, please contact Stefan Feske, MD, program director, at stefan.feske@nyulangone.org or William A. Coetzee, PhD, core facility director, at william.coetzee@nyulangone.org with details regarding your anticipated needs.

Manual Patch Clamp Setup

The core facility is equipped with a manual patch clamp setup that includes an Olympus IX71 inverted microscope with fluorescent Xenon light source; a Molecular Devices Axon™ Axopatch™ 200B-2 ultra-low-noise patch clamp amplifier; a Molecular Devices 16-bit digidata 1440 low noise data acquisition system; a Molecular Devices pCLAMP™ 10 data acquisition and analysis software suite; a Parker Precision Fluidics micromanipulator system (including two MP-285 manipulator mechanicals, one MPC-200 controller, and one ROE-200 control unit); a Parker Precision Fluidics Picospritzer III liquid application microinjector; and an AutoMate Scientific ValveLink8® Perfusion system.

CytoPatch Automated Patch Clamp System

Investigators have access to a Cytocentrics CytoPatch™ four-channel automated patch clamp system designed for acquiring high-fidelity patch clamp data at medium to high-throughput.

Specific features include the ability to simultaneously patch clamp four individual cells; a microfluidic device which allows for the formation of giga-ohm seals; a fully-integrated liquid handling system that enables extremely fast solution exchange (4 ms washin, 10 ms washout); no need for special sealing and “seal enhancing” solutions, thus avoiding the toxic effects of F– and Ca2+ on cell function and ion channel activity; and air temperature control within the instrument and separate temperature control of the recording position. The unit works with multiple cell types (cell lines, stem cells and derivatives, and primary cells) and channel types.

See additional information about the automated patch clamp system.

FlexStation 3 Microplate Reader

Molecular Devices’ FlexStation 3 microplate reader allows for measurements of fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Programmable liquid handling allows for automated ratiometric, time-lapse measurements of ion concentrations in cells and organelles.

See additional information about the FlexStation 3 microplate reader.

Accessory Equipment

Accessory equipment includes a Sutter micropipette puller P-97; an osmometer; an electrolyte analyzer; a tissue culture hood; a centrifuge; and an incubator for mammalian cell culture.