![""](/research/scientific-cores-shared-resources/sites/default/files/styles/fixed_height_250_1600w_320h/public/2022-06/scientific-cores-cytometry-and-cell-sorting-laboratory-hero_6.jpg?h=2c7ac911&itok=EOTvU_lt)
FACSAria II Self-Service
The expert staff at NYU Langone’s Cytometry and Cell Sorting Laboratory provide researchers with access to a FACSAria II self-service flow cytometer. Researchers who wish to reserve time on this instrument must first complete required training.
Reserve this instrument using iLab (Kerberos ID and password required).
![FACSAria II Self-Service Flow Cytometer](/research/scientific-cores-shared-resources/sites/default/files/cytometry/BD-FACSAria-I-Self-Service.jpg)
FACSAria II Location
NYU Langone
Joan and Joel Smilow Research Center
522 First Avenue, Room 609B
Phone: 212-263-5907
FACSAria II Optics
4 lasers and 15 colors (see configuration)
FACSAria II Biosafety Level
- BSL-2 (unscreened human material or infectious material)
- mandatory advance notice for setup and decontamination for infectious sorts
- BSL-2 standard operating procedure
FACSAria II Pricing
- $49 per hour: Perlmutter Cancer Center users
- $52 per hour: non–Perlmutter Cancer Center users
- $77 per hour: external academic users
- $103 per hour: external industry users
FACSAria II Sample Preparation
- Acceptable sample tubes are polypropylene 12 × 75 mm, 15-mL tubes, bullet tubes, or 1.5-mL microcentrifuge tubes.
- Concentration should be 10–20 × 106 cells per mL (allows for ideal pressure and best use of time).
- Always bring extra media to dilute if necessary.
- Filter all samples as close to sorting time as possible.
FACSAria II Sample Collection
Collection tubes should be coated with appropriate media (such as FBS):
- 4 5-mL (12 × 75 mm) tubes
- 2 15-mL tubes
- 4 1.5-mL microcentrifuge tubes
- well plates (up to 384 wells)
- 4 “bullet” tubes (1-mL tubes)
- 4 polymerase chain reaction (PCR) tubes
- slides and cover slips
FACSAria II Nozzle Options
70 µm
This is the default nozzle size.
- This nozzle can sort 7.6 × 107 total cells per hour; collection volume is ~106 cells in 1 mL.
- Requires higher pressure (70 psi) and is not ideal for larger or fragile cells.
100 µm
This is not the default nozzle size; it must be changed back to 70 μm when done.
- It can sort 3.3 × 107 cells per hour; collection volume is ~4 × 105 cells in 1 mL.
- It is best for large or delicate cells because it uses lower pressure (20 psi).
- This nozzle is appropriate for stem cells, fibroblasts, neurons, and larger cancer cells.
FACSAria II Tutorials
FACSAria II Self-Serve Quick Guide
FACSAria II Configuration
Self-Service Aria II (5 laser, 18 parameters)
Review the configuration of the FACSAria II below, or access a printable document.