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Cytometry & Cell Sorting Laboratory Instruments & Services Synergy

Synergy

The expert staff at NYU Langone’s Cytometry and Cell Sorting Laboratory can assist you in using a Synergy cell sorter. Reserve this instrument using iLab.

Synergy.jpg

Synergy Location

NYU Langone
BSL-3 Lab

Synergy Optics

3 lasers and 10 colors (see configuration)

Synergy Biosafety Level

  • dedicated BSL-3 sorting
  • sorted cells treated as BSL-3 material

Synergy Pricing

  • $258 per hour: Perlmutter Cancer Center users
  • $258 per hour: non–Perlmutter Cancer Center users
  • $438 per hour: external academic users
  • $464 per hour: external industry users

Synergy Sample Preparation

  • Use only 12 × 75 mm sample tubes (polypropylene is recommended).
  • Concentration should be 10–20 × 106 cells/mL (allows for ideal pressure and best use of time).
  • Always bring extra media to dilute if necessary.
  • Filter all samples as close to sorting time as possible.

Synergy Sample Collection

Collection tubes should be coated with appropriate media (such as FBS):

  • 4 5-mL (12 × 75 mm) tubes
  • 1 50-mL tube
  • 2 15-mL tubes
  • 4 1.5-mL microcentrifuge tubes
  • slides and cover slips

Synergy Nozzle options

70 µm

  • This nozzle can sort 9 × 107 total cells per hour; collection volume is ~106 cells in 1 mL.
  • Because it requires higher pressure (70 psi), it is not ideal for larger or fragile cells.

100 µm

  • This nozzle can sort 5.4 × 107 cells per hour; collection volume is ~4 × 105 cells in 1 mL.
  • It is best for large or delicate cells because it uses lower pressure (20 psi).
  • Examples include stem cells, fibroblasts, neurons, and larger cancer cells.
  • Thirty minutes’ extra setup time is required.

Synergy Configuration

Review the configuration of the Synergy below, or access a printable document

Synergy Config