
ZE5 Analyzer
The expert staff at NYU Langone’s Cytometry and Cell Sorting Laboratory provides researchers with training on and access to a ZE5 analyzer. Reserve this instrument using iLab (Kerberos ID and password required).

ZE5 Analyzer Location
We provide two instruments at the following locations:
NYU Langone
Alexandria Center for Life Science
430 East 29th Street
West Tower, Room 329
NYU Langone
Science Building
435 East 30th Street, Room 449
ZE5 Analyzer Optics
5 lasers and 27 parameters (see configuration)
ZE5 Analyzer Level
- BSL-2 (unscreened human material or infectious material)
- BSL-2 standard operating procedure
ZE5 Analyzer Sample Preparation
- Sample tubes: use only 12 x 75 mm tubes, variety of well plates.
- Concentration should be 1–5 × 106 cells/mL.
- Always bring extra media to dilute if necessary.
- Filter large or sticky cells.
ZE5 Analyzer Tutorials
Everest Software Offline Setup
Manually Adjusting the Scaling of ZE5 Data in FlowJo
ZE5 Analyzer Configuration
Review the configuration of the ZE5 Analyzer below, or access a printable document.