ZE5 Analyzer | NYU Langone Health

Cytometry & Cell Sorting Laboratory Instruments & Services ZE5 Analyzer

ZE5 Analyzer

The expert staff at NYU Langone’s Cytometry and Cell Sorting Laboratory provides researchers with training on and access to a ZE5 analyzer. Reserve this instrument using iLab (Kerberos ID and password required).

ZE5 Yeti
NYU Langone’s Cytometry and Cell Sorting Laboratory offers a ZE5  analyzer for investigators.

ZE5 Analyzer Location

We provide two instruments at the following locations:

NYU Langone
Alexandria Center for Life Science
430 East 29th Street
West Tower, Room 329

NYU Langone
Science Building
435 East 30th Street, Room 449

ZE5 Analyzer Optics

5 lasers and 27 parameters (see configuration)

ZE5 Analyzer Level

ZE5 Analyzer Sample Preparation

  • Sample tubes: use only 12 x 75 mm tubes, variety of well plates.
  • Concentration should be 1–5 × 106 cells/mL.
  • Always bring extra media to dilute if necessary.
  • Filter large or sticky cells.

ZE5 Analyzer Tutorials

ZE5 user guide

Everest Software Offline Setup

Manually Adjusting the Scaling of ZE5 Data in FlowJo

 

ZE5 Analyzer Configuration

Review the configuration of the ZE5 Analyzer below, or access a printable document.

NYU Langone DART Cytometry and Cell Sorting Laboratory’s ZE5 5 laser (488nm, 561nm, 405nm, 355nm, 640nm) configuration.

NYU Langone DART Cytometry and Cell Sorting Laboratory’s ZE5 filter set up.