The expert staff at NYU Langone’s Cytometry and Cell Sorting Laboratory can train and certify you to operate our SH800Z cell sorter. This instrument offers 6 color measurements from 4 colinear lasers and can sort at a rate 5,000 events per second, with 99 percent purity. A user-supplied chip allows for automated setup and minimal cross-contamination.

Reserve this instrument using iLab (Kerberos ID and password required).

SH800Z Cell Sorter Location

NYU Langone
Science Building
435 East 30th Street, Room 449

SH800Z Cell Sorter Optics

4 laser and 6 colors (see configuration )

SH800Z Cell Sorter Biosafety Level

• BSL-2 (unscreened human material or infectious material)
• Set-up and decontamination for infectious sorts are the user’s responsibility
• BSL-2 standard operating procedure

SH800Z Cell Sorter Pricing

• $49 per hour: Perlmutter Cancer Center users
• $52 per hour: non–Perlmutter Cancer Center users
• $77 per hour: external academic users
• $103 per hour: external industry users

In addition, use of this instrument requires a sorting chip. This can be obtained from the laboratory for $41 each or directly from Sony in boxes of 40.

SH800Z Cell Sorter Sample Preparation

• Use only 12 x 75 mm sample tubes (polypropylene is recommended), 1.5mL eppendorfs, or 15cc conical tubes.
• Concentration should be 10–20 × 10^6 cells/mL (allows for ideal pressure and best use of time).
• Always bring extra media to dilute if necessary.
• Filter all samples as close to sorting time as possible.

SH800Z Cell Sorter Sample Collection

Collection tubes should be coated with appropriate media (such as fetal bovine serum, or FBS):

• 2 5-mL (12 × 75 mm) tubes
• 2 1.5mL centrifuge tubes
• 2 15cc conical tubes
• microplates (up to 384 wells each)

SH800Z Cell Sorter Nozzle Options

70µm

• Can sort 4 × 10^7 total cells per hour, collection volume: ~10^6 cells in 1mL
• Requires higher pressure (60 psi), not ideal for larger or fragile cells

100µm

• Can sort 2 × 10^7 cells/hour, collection volume is ~2.5 × 10^5 cells in 1 mL
• Best for large or delicate cells because it uses lower pressure (20psi)
• Examples include stem cells, fibroblasts, neurons, and larger cancer cells

SH800z Cell Sorter Tutorials

SH800z Sorter Sony Quick Guide
SH800z Sorter Laboratory Quick Guide
SH800z Consumable Ordering Info

SH800Z Cell Sorter Configuration

Review the configuration of the SH800Z Cell Sorter below, or access a printable document.