The expert staff at NYU Langone Health’s Cytometry and Cell Sorting Laboratory can assist you in using the Bigfoot Spectral cell sorter.

Reserve this cell sorter using iLab (Kerberos ID and password required).

Bigfoot Spectral Cell Sorter Location

NYU Langone Health
550 First Avenue
Smilow Cellar, Room C30
Phone: 212-263-5907

Bigfoot Spectral Cell Sorter Optics

7 lasers (349nm, 405nm, 445nm, 488nm, 561nm, 640nm, 785nm) and 30+ colors. Learn more about Bigfoot configuration.

Bigfoot Spectral Cell Sorter Biosafety Level

• BSL-2+ (unscreened human material or infectious material)
• Mandatory advance notice for setup and decontamination of infectious sorts
• BSL-2+ standard operating procedure

Bigfoot Spectral Cell Sorter Sample Preparation

• Acceptable sample tubes are polypropylene 12 × 75 mm tubes, 15-mL tubes, bullet tubes, or 1.5-mL microcentrifuge tubes.
• Concentration should be 10–20 × 10⁶ cells/mL (allows for ideal pressure and best use of time).
• Always bring extra media to dilute if necessary.
• Filter all samples as close to sorting time as possible.

Bigfoot Spectral Cell Sorter Sample Collection

• Up to 6  1.5ml tubes
• Up to 6  5ml tubes
• Up to 4  15ml tubes
• 8 well PCR strips (10X)
• 6, 12, 24, 96, 384, 1536 well plates
• 96 and 384 deep well plates
• 1 50ml tube with: 3 1.5ml tubes, 3 5ml tubes, or 2 15ml tubes
• Bulk sorting: 6 tube sample loader, up to 3 sort output sets
• Multi-stream plate sort modes (up to 4 streams)

Bigfoot Spectral Cell Sorter Nozzle Options

3 Nozzle sizes available (jet-in-air):
70 um (60psi), 100 um (30 psi), 120 um (20 psi)

Bigfoot Spectral Cell Sorter Filter Setup

Bigfoot Spectral Cell Sorter Configuration

Learn more about Bigfoot configuration.