The expert staff at NYU Langone’s Cytometry and Cell Sorting Laboratory can assist you in using either or both of our SY3200™ highly automated parallel sorting (HAPS) cell sorters. If you plan to reserve both independently operated instruments—SY3200™ HAPS 1 and HAPS 2—simultaneously, please allow for twice the setup time and control amounts.
Reserve this instrument using iLab.
540 First Avenue
Skirball Institute of Biomolecular Medicine
3rd Floor, Lab 9
4 lasers and 10 colors (see configuration)
Note that SY3200™ HAPS 1 and HAPS 2 are two independent instruments with identical technical capabilities.
SY3200 Biosafety Level
- BSL-2 (unscreened human material or infectious material)
- mandatory advance notice for setup and decontamination for infectious sorts
- BSL-2 standard operating procedure
- $144 per hour: Perlmutter Cancer Center users
- $155 per hour: non–Perlmutter Cancer Center users
- $206 per hour: external academic users
- $258 per hour: external industry users
SY3200 Sample Preparation
- Use only 12 × 75 mm sample tubes (polypropylene is recommended).
- Concentration should be 10–20 × 106 cells per mL (allows for ideal pressure and best use of time).
- Always bring extra media to dilute if necessary.
- Filter all samples as close to sorting time as possible.
SY3200 Sample Collection
Collection tubes should be coated with appropriate media (such as FBS):
- 4 5-mL (12 × 75 mm) tubes
- 1 50-mL tube
- 2 15-mL tubes
- 4 1.5-mL microcentrifuge tubes
- slides and cover slips
- well plates (up to 384 wells)
SY3200 Nozzle Options
- Both HAPS 1 and HAPS 2 are set up with 100µm nozzles by default.
- This nozzle can sort 4.5 × 107 cells per hour; collection volume is ~4 × 105 cells in 1 mL.
- It is best for large or delicate cells, because it uses lower pressure (25 psi).
- Examples include stem cells, fibroblasts, neurons, and larger cancer cells.