The expert staff at NYU Langone’s Cytometry and Cell Sorting Laboratory can assist you in using a MoFlo XDP cell sorter. Reserve this instrument using iLab (Kerberos ID and password required).

MoFlo XDP Location

NYU Langone
540 First Avenue
3rd Floor, Lab 9
Phone: 212-263-5907

MoFlo XDP Optics

4 lasers and 11 colors (see configuration)

MoFlo XDP Biosafety Level

• BSL-2 (unscreened human material or infectious material)
• mandatory advance notice for setup and decontamination for infectious sorts
• BSL-2 standard operating procedure

MoFlo XDP Pricing

• $144 per hour: Perlmutter Cancer Center users
• $155 per hour: non–Perlmutter Cancer Center users
• $206 per hour: external academic users
• $258 per hour: external industry users

MoFlo XDP Sample Preparation

• Use only 12 × 75 mm sample tubes (polypropylene is recommended).
• Concentration should be 10–20 × 10⁶ cells/mL (allows for ideal pressure and best use of time).
• Always bring extra media to dilute if necessary.
• Filter all samples as close to sorting time as possible.

MoFlo XDP Sample Collection

Collection tubes should be coated with appropriate media (such as fetal bovine serum, or FBS):

• 4 5-mL (12 × 75 mm) tubes
• 4 1.5-mL microcentrifuge tubes
• slides and cover slips
• well plates (up to 384 wells each)

MoFlo XDP Nozzle Options

70 µm

• This nozzle can sort 8.8 × 10⁷ total cells per hour; collection volume is ~10⁶ cells in 1 mL.
• It requires higher pressure (60 psi) and is not ideal for larger or fragile cells.

100 µm

• This nozzle can sort 4.5 × 10⁷ cells per hour; collection volume is ~4 × 10⁵ cells in 1 mL.
• It is best for large or delicate cells because it uses lower pressure (30 psi).
• Examples include stem cells, fibroblasts, neurons, and larger cancer cells.
• It requires 30 minutes’ extra setup time.

MoFlo XDP Configuration

Review the configuration of the MoFlo XDP below, or access a printable document.