The expert staff at NYU Langone’s Cytometry and Cell Sorting Laboratory provide researchers with access to a FACSAria II self-service flow cytometer. Researchers who wish to reserve time on this instrument must first complete required training.

Reserve this instrument using iLab (Kerberos ID and password required).

FACSAria II Location

NYU Langone
Joan and Joel Smilow Research Center
522 First Avenue, Room 609B
Phone: 212-263-5907

FACSAria II Optics

4 lasers and 15 colors (see configuration)

FACSAria II Biosafety Level

• BSL-2 (unscreened human material or infectious material)
• mandatory advance notice for setup and decontamination for infectious sorts
• BSL-2 standard operating procedure

FACSAria II Pricing

• $49 per hour: Perlmutter Cancer Center users
• $52 per hour: non–Perlmutter Cancer Center users
• $77 per hour: external academic users
• $103 per hour: external industry users

FACSAria II Sample Preparation

• Acceptable sample tubes are polypropylene 12 × 75 mm, 15-mL tubes, bullet tubes, or 1.5-mL microcentrifuge tubes.
• Concentration should be 10–20 × 10⁶ cells per mL (allows for ideal pressure and best use of time).
• Always bring extra media to dilute if necessary.
• Filter all samples as close to sorting time as possible.

FACSAria II Sample Collection

Collection tubes should be coated with appropriate media (such as FBS):

• 4 5-mL (12 × 75 mm) tubes
• 2 15-mL tubes
• 4 1.5-mL microcentrifuge tubes
• well plates (up to 384 wells)
• 4 “bullet” tubes (1-mL tubes)
• 4 polymerase chain reaction (PCR) tubes
• slides and cover slips

FACSAria II Nozzle Options

70 µm

This is the default nozzle size.

• This nozzle can sort 7.6 × 10⁷ total cells per hour; collection volume is ~10⁶ cells in 1 mL.
• Requires higher pressure (70 psi) and is not ideal for larger or fragile cells.

100 µm

This is not the default nozzle size; it must be changed back to 70 μm when done.

• It can sort 3.3 × 10⁷ cells per hour; collection volume is ~4 × 10⁵ cells in 1 mL.
• It is best for large or delicate cells because it uses lower pressure (20 psi).
• This nozzle is appropriate for stem cells, fibroblasts, neurons, and larger cancer cells.

FACSAria II Tutorials

FACSAria II Self-Serve Quick Guide

FACSAria II Configuration

Self-Service Aria II (5 laser, 18 parameters)

Review the configuration of the FACSAria II below, or access a printable document.

Self-Service Aria II Filter Setup