Ion Laboratory Instruments
NYU Langone’s Ion Laboratory provides investigators with access to electrophysiology and other instrumentation to measure ion channel and transporter function.
Manual Patch Clamp System
The IonLab is equipped with a manual patch clamp setup composed of an Olympus BX51WI upright microscope equipped for IR-DIC illumination and a fluorescent xenon light source; a Molecular Devices Axon Axopatch 200B ultra-low-noise patch clamp amplifier; a Molecular Devices 16-bit Digidata 1550A low-noise data acquisition system; a Molecular Devices pCLAMP 10 data acquisition and analysis software suite; a Sutter Instrument micromanipulator system (including one MP-285 manipulator mechanical, one MPC-200 controller, and one ROE-200 control unit); and an AutoMate Scientific ValveLink8 perfusion system. Overflow capacity is available using the patch clamp system in the lab of William A. Coetzee, DSc.
The Nanion Patchliner is a fully automated planar patch clamp instrument that can record as many as eight cells simultaneously, allowing for versatile basic research of ion channel biophysics and mechanisms of action. It enables routine and sophisticated assays such as heat activation of transient receptor potential channels, activation of Ca2+-activated channels by internal exchange, and fast external solution exchange with minimal exposure for ligand-gated ion channels.
See additional information about the Nanion Patchliner.
Sophion QPatch II
The Sophion QPatch II is an automated patch clamp system. Its intuitive software interface and hardware design deliver robust unattended operation. It features no-seal enhancing agents, individual pipetting, and individual control of pressure systems, ensuring that experiments perform close to physiological conditions and that all wells are handled individually.
See additional information about the Sophion QPatch II.
FlexStation 3 Microplate Reader
The Molecular Devices FlexStation 3 microplate reader allows for measurements of fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Programmable liquid handling allows for automated ratiometric, time-lapse measurements of the concentrations of ions such as Ca2+, Mg2+, Zn2+, and others in cells and organelles.
See additional information about the FlexStation 3 microplate reader.
SURFE2R N1 Surface Electrogenic Event Reader
Currently located in the laboratory of David L. Stokes, PhD, the Nanion SURFE2R N1 is based on solid-supported membrane (SSM) technology and designed for measuring the activity of electrogenic transporters (symporters, exchangers, and uniporters) and pumps.
See additional information about the SURFE2R N1 surface electrogenic event reader.
Advanced Micro-Osmometer Model 3300
The Advanced Instruments Advanced micro-osmometer model 3300 determines the osmolality of solutions using freezing point depression (FPD). It is ideal for serum, urine, or other bodily fluids in clinical applications; and media or formulations in pharmaceutical, biotech or industrial applications. It is automated, single sample, and designed to process a 20-µL sample in a 1-minute test time, allowing the model 3300 to provide the best accuracy and precision.
See additional information about the Advanced micro-osmometer model 3300.
SmartLyte PLUS Electrolyte Analyzer
The Diamond Diagnostics SmartLyte PLUS electrolyte analyzer is a completely automated system measuring Na+, K+, Cl−, Ca2+, and Li+ in whole blood, serum, plasma, urine, media, and formulations utilizing ion-selective electrode (ISE) technology.
See additional information about the SmartLyte PLUS electrolyte analyzer.
EVOS M5000 Microscope
The Thermo-Scientific EVOS M5000 microscope delivers publication-ready images with a user-friendly workflow. It is equipped with a high-resolution CMOS camera, the ability to capture three fluorescence channels (DAPI, GFP, and Texas Red) plus transmitted light.
See additional information about the EVOS M5000 Microscope.
Accessory equipment available in the IonLab includes an Eppendorf refrigerated microcentrifuge, a tissue culture hood, a Thermo incubator for mammalian cell culture, and refrigerators to maintain and prepare cells while conducting experiments in the facility.